fgf20 is essential for initiating zebrafish fin regeneration
fgf20 is essential for initiating zebrafish fin regeneration
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DOI：
10.1126/science.1117637
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发表时间：
2005-12-23
期刊：
影响因子：
56.9
通讯作者：
Keating, MT
中科院分区：
文献类型：
--
作者：
Whitehead, GG;Makino, S;Keating, MT
表型再生需要存在或创造能够再生失去的器官的多能细胞。斑马鱼鳍的再生是由芽基细胞的产生介导的。在这里，我们描述了缺乏芽基（dob）突变体，在初始步骤中鳍再生失败，形成异常再生上皮，并且不形成芽基。这种突变对胚胎存活没有影响。Dob由fgf 20 a无效突变Y148 S引起。fgf 20 a在鳍再生的起始期间在上皮-间充质边界处表达，并且随后与芽基标记msxb重叠。因此，fgf 20 a具有再生特异性要求，启动鳍再生，并控制芽基形成。
Epimorphic regeneration requires the presence or creation of pluripotent cells capable of reproducing lost organs. Zebrafish fin regeneration is mediated by the creation of blastema cells. Here, we characterize the devoid of blastema (dob) mutant that fails fin regeneration during initial steps, forms abnormai regeneration epithelium, and does not form blastema. This mutation has no impact on embryonic survival. Dob results from an fgf20a null mutation, Y148S. Fgf20a is expressed during initiation of fin regeneration at the epithelial-mesenchymal boundary and later overlaps with the blastema marker msxb. Thus, fgf20a has a regeneration-specific requirement, initiating fin regeneration, and controlling blastema formation.