Real-time quantitative PCR, pathogen detection and MIQE.
Real-time quantitative PCR, pathogen detection and MIQE.
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DOI：
10.1007/978-1-60327-353-4_1
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Bustin, Stephen A
中科院分区：
文献类型：
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作者：
Johnson, Gemma;Nolan, Tania;Bustin, Stephen A
核酸是最终的生物标志物，实时PCR（qPCR）已被确定为核酸检测的首选方法。它们共同实现了病原体的准确、灵敏和特异性鉴定，qPCR的使用已成为诊断实验室的常规。基于qPCR的检测的可靠性依赖于最佳样品选择、检测设计和验证以及适当的数据分析的组合，而“实时PCR出版物的最小信息”（MIQE）指南旨在提高检测设计的可靠性以及报告的透明度，这是qPCR保持分子诊断基准技术的必要条件。
Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination of optimal sample selection, assay design and validation as well as appropriate data analysis and the "Minimal Information for the Publication of real-time PCR" (MIQE) guidelines aim to improve both the reliability of assay design as well as the transparency of reporting, essential conditions if qPCR is to remain the benchmark technology for molecular diagnosis.