Purine nucleotides modulate proliferation of brown fat preadipocytes
Purine nucleotides modulate proliferation of brown fat preadipocytes
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DOI：
10.1046/j.1365-2184.1999.32230131.x
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发表时间：
1999-04-01
影响因子：
8.5
通讯作者：
Pappone, PA
中科院分区：
文献类型：
--
作者：
Wilson, SM;Barsoum, MJ;Pappone, PA
使用培养的分离的大鼠肩胛间棕色脂肪前脂肪细胞测试了嘌呤核苷酸和核苷影响棕色脂肪前脂肪细胞增殖的假设。每天向培养物中添加 100 μM 三磷酸腺苷 (ATP) (n = 4)，与使用 CyQUANT-GR 荧光测量的对照培养物相比 (P < 0.05)，相对 DNA 含量提高了 1.5 倍。较高浓度的 ATP 抑制生长，500 (n = 2) 或 1000 mu M ATP (n = 3) 几乎完全抑制生长。相对于对照培养物，ATP (100 μM) 没有影响，而 250-1000 CIM ATP 降低了蛋白质含量。腺苷（100μM；n=3）不影响DNA或蛋白质含量，但500μM和1000μM腺苷抑制棕色脂肪细胞增殖并抑制蛋白质合成。通过荧光素-荧光素酶生物发光测定，培养的棕色脂肪细胞快速去除或降解培养基中的 ATP，这表明高 ATP 浓度的抑制作用可能是由于其分解为腺苷所致。结果支持ATP促进棕色脂肪细胞增殖而腺苷抑制棕色脂肪细胞增殖的结论。
The hypothesis that purine nucleotides and nucleosides affect brown fat preadipocyte proliferation was tested using isolated rat interscapular brown fat preadipocytes in culture. Daily addition of 100 mu M adenosine triphosphate (ATP) (n = 4) to cultures enhanced the relative DNA content by 1.5-fold compared to control cultures (P < 0.05) measured using CyQUANT-GR fluorescence. Higher concentrations of ATP inhibited growth and 500 (n = 2) or 1000 mu M ATP (n = 3) almost completely inhibited growth. ATP (100 mu M) did not affect while 250-1000 CIM ATP decreased protein content relative to control cultures. Adenosine (100 mu M; n = 3) did not affect DNA or protein content, but 500 mu M and 1000 mu M adenosine suppressed brown adipocyte proliferation and inhibited protein synthesis. Cultured brown adipocytes quickly removed or degraded ATP in the culture media as determined by luciferin-luciferase bioluminescence, suggesting that the inhibitory effects of high ATP concentrations may result from its breakdown to adenosine. The results support the conclusion that ATP promotes and adenosine inhibits brown adipocyte proliferation.