Combined non-steroidal therapy in experimental corneal injury
Combined non-steroidal therapy in experimental corneal injury
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DOI：
10.1159/000268009
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发表时间：
1997-05-01
影响因子：
2.1
通讯作者：
RuizMoreno, JM
中科院分区：
文献类型：
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作者：
Laria, C;Alio, JL;RuizMoreno, JM
目的：研究了抗炎性非甾体类药物联合自由基清除剂治疗实验性角膜损伤的效果，并与皮质类固醇治疗进行了比较。方法：新西兰白化病兔80只。通过在右角膜的中心轴上施加1-N NaOH滤纸30 s来诱导角膜碱烧伤。将动物分为5个治疗组：第1组（对照组）仅给予庆大霉素;第2组给予0.5%二甲基硫脲（DMU）;第3组给予1%地塞米松;第4组给予0.5% DMU和1%吲哚美辛联合给药;第5组给予0.5% DMU和0.1%双氯芬酸钠。每12小时滴入一滴50 μ l庆大霉素，而其他药物每6小时滴入一滴（50 μ l）。所有组均接受与对照组相同的抗生素治疗。动物在第五天被杀死。观察角膜炎症指数、角膜损伤区面积、周长及角膜透明度。结果：72 h后，治疗组与对照组的炎症指标无显著性差异。当与对照组相比时，在第3-5组中在24小时观察到显著差异（p < 0.001）。平面测量显示，第4组与其他组相比有显著差异（p < 0.05）。角膜透明度研究显示，与包括第3组在内的其他组相比，第4组和第5组的值在统计学上显著更好（p < 0.05）。结论：在我们的实验性化学性角膜损伤中，使用0.5% DMU联合1%吲哚美辛可被认为是皮质类固醇治疗的替代方案。
Purpose: The effects of anti-inflammatory non-steroidal therapy combined with free-radical scavengers were studied and compared to corticosteroid use in the treatment of experimental corneal injury. Method: Eighty New Zealand albino rabbits were used in this study. A corneal alkali burn was induced by applying 1-N NaOH filter paper on the central axis of the right cornea for 30 s. Animals were distributed into five treatment groups: group 1 (control group) was only given gentamicin; group 2 was treated with 0.5% dimethylthiourea (DMU); group 3 received 1% dexamethasone; group 4 was given combined 0.5% DMU and 1% indomethacin; group 5 was treated with 0.5% DMU and 0.1% diclofenac sodium. One 50-mu l drop of gentamicin was instilled every 12 h, whereas the other drugs were instilled every 6 h (50 mu l). All groups received the same antibiotic treatment as the control group. The animals were killed on the 5th day. Inflammatory index, area and perimeter of the wounded corneal zone, and corneal transparency were evaluated. Results: No significant differences in the inflammatory index were found between the treatment groups and the control group after 72 h. Significant differences (p < 0.001) were observed at 24 h in groups 3-5 when compared with the control group. Planimetry showed significant differences in group 4 when compared with the other groups (p < 0.05). Corneal transparency study showed statistically significantly better values in groups 4 and 5, when compared with the other groups, including group 3 (p < 0.05). Conclusions: The use of 0.5% DMU combined with 1% indomethacin can be considered an alternative to corticosteroid treatment in our experimental chemical corneal injury.