Transient electrical coupling regulates formation of neuronal networks.
Transient electrical coupling regulates formation of neuronal networks.
复制标题
瞬态电耦合调节神经元网络的形成。
DOI：
10.1016/j.brainres.2006.09.112
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发表时间：
2007
期刊：
影响因子：
2.9
通讯作者：
Zoran,MarkJ
中科院分区：
文献类型：
--
作者：
Szabo,TheresaM;Zoran,MarkJ
在化学突触强烈形成的发育窗口之前和期间，电突触丰富，因此可能有助于神经元网络的建立。在神经损伤后的体内和体外 48-72 小时内，再生的 Helisoma 运动神经元 110 和 19 之间会产生瞬时电耦合，然后被消除。这些突触处的电耦合和化学突触传递之间存在反比关系，使得电耦合的下降与胆碱能突触传递的出现同时发生。在这项研究中，我们生成了两细胞和三细胞神经元网络，以测试预测的突触生成能力是否受到先前突触相互作用的影响。电生理学分析表明，三细胞神经元网络中形成的突触并不是根据两细胞网络中的突触发生结果预测的。因此，神经元网络内新的电和化学突触的形成取决于该网络的现有连接性。此外，与现有网络形成的新联系对这些现有联系几乎没有影响。这些结果表明，网络依赖性机制，特别是由间隙连接耦合介导的机制，调节简单神经网络内的突触形成。
Electrical synapses are abundant before and during developmental windows of intense chemical synapse formation, and might therefore contribute to the establishment of neuronal networks. Transient electrical coupling develops and is then eliminated between regenerating Helisoma motoneurons 110 and 19 during a period of 48–72 h in vivo and in vitro following nerve injury. An inverse relationship exists between electrical coupling and chemical synaptic transmission at these synapses, such that the decline in electrical coupling is coincident with the emergence of cholinergic synaptic transmission. In this study, we have generated two- and three-cell neuronal networks to test whether predicted synaptogenic capabilities were affected by previous synaptic interactions. Electrophysiological analyses demonstrated that synapses formed in three-cell neuronal networks were not those predicted based on synaptogenic outcomes in two-cell networks. Thus, new electrical and chemical synapse formation within a neuronal network is dependent on existing connectivity of that network. In addition, new contacts formed with established networks have little impact on these existing connections. These results suggest that network-dependent mechanisms, particularly those mediated by gap junctional coupling, regulate synapse formation within simple neural networks.