Active screen plasma surface modification of polycaprolactone to improve cell attachment
Active screen plasma surface modification of polycaprolactone to improve cell attachment
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DOI：
10.1002/jbm.b.31916
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发表时间：
2012-02-01
影响因子：
3.4
通讯作者：
Dong, Hanshan
中科院分区：
文献类型：
--
作者：
Fu, Xin;Sammons, Rachel L.;Dong, Hanshan
为了定制聚己内酯（PCL）的表面性能，用于生物医学应用，PCL薄膜样品的表面改性的活性屏等离子体氮化（ASPN）技术。用傅里叶变换红外光谱和X射线光电子能谱对产物的化学组成和结构进行了表征。用接触角法和表面能法研究了表面改性聚合物的润湿性。使用MC 3 T3-E1成骨细胞样细胞体外评价制备的PCL样品的生物相容性。通过测定自降解速率（由脂肪酶催化）来评估降解性。结果表明，ASPN表面改性能有效地提高成骨细胞在PCL表面的粘附和铺展。化学组成的主要变化是表面上的一些羧基交换为羟基。活性屏等离子渗氮技术是一种有效提高成骨细胞在PCL表面粘附和铺展的实用方法。这种变化归因于等离子体处理增加了润湿性并产生了新的羟基。活性屏等离子体处理后的PCL膜仍具有生物降解性，但酶降解速率比未处理的PCL膜慢。(C)2011 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 100 B：314320，2012.
To tailor polycaprolactone (PCL) surface properties for biomedical applications, film samples of PCL were surface modified by the active screen plasma nitriding (ASPN) technique. The chemical composition and structure were characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The wettability of the surface modified polymers was investigated by contact angle and surface energy methods. Biocompatibility of the prepared PCL samples was evaluated in vitro using MC3T3-E1 osteoblast-like cells. The degradability was assessed by determining the self-degradation rate (catalyzed by lipase). The results show that ASPN surface modification can effectively improve osteoblast cell adhesion and spreading on the surface of PCL. The main change in chemical composition is the exchange of some carboxyl groups on the surface for hydroxyl groups. The active-screen plasma nitriding technique has been found to be an effective and practical method to effectively improve osteoblast cell adhesion and spreading on the PCL surface. Such changes have been attributed to the increase in wettablity and generation of new hydroxyl groups by plasma treatment. After active-screen plasma treatment, the PCL film is still degradable, but the enzymatic degradation rate is slower compared with untreated PCL film. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 314320, 2012.