Making gene drive biodegradable.
Making gene drive biodegradable.
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DOI：
10.1098/rstb.2019.0804
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发表时间：
2021-02-15
期刊：
影响因子：
--
通讯作者：
Adelman ZN
中科院分区：
文献类型：
--
作者：
Zapletal J;Najmitabrizi N;Erraguntla M;Lawley MA;Myles KM;Adelman ZN
长期以来，人们一直在寻找基因驱动系统来改变蚊子种群，从而抗击疟疾和登革热。强大的基因驱动系统已经在实验室实验中开发出来，但可能永远不会在实践中使用，除非它们能通过严格的现场测试证明是可以接受的。由于从自然界中移除基因驱动转基因的预期困难，这种测试变得复杂起来。在这里，我们考虑将自我消除机制纳入基于归巢的基因驱动转基因的设计中。这种方法不仅导致了基因驱动转基因的切除，而且产生了一个抵抗基因驱动进一步作用的非转基因等位基因。引人注目的是，我们的模型表明，这种机制以适度的速度(10%)作为单组分系统的一部分，将足以导致即使是最强大的基于归巢的基因驱动转基因的快速逆转，而不需要进一步的补救。模型还表明，与基因驱动转基因本身不同，自我消除转基因方法预计可以容忍相当高的失败率。因此，自我消除技术可以通过对自然界中存在的基因驱动转基因建立严格的时间限制，使它们基本上可生物降解，从而允许对基因驱动进行严格的现场测试。这篇文章是“蚊媒疾病的新控制策略”主题的一部分。
Gene drive systems have long been sought to modify mosquito populations and thus combat malaria and dengue. Powerful gene drive systems have been developed in laboratory experiments, but may never be used in practice unless they can be shown to be acceptable through rigorous field-based testing. Such testing is complicated by the anticipated difficulty in removing gene drive transgenes from nature. Here, we consider the inclusion of self-elimination mechanisms into the design of homing-based gene drive transgenes. This approach not only caused the excision of the gene drive transgene, but also generates a transgene-free allele resistant to further action by the gene drive. Strikingly, our models suggest that this mechanism, acting at a modest rate (10%) as part of a single-component system, would be sufficient to cause the rapid reversion of even the most robust homing-based gene drive transgenes, without the need for further remediation. Modelling also suggests that unlike gene drive transgenes themselves, self-eliminating transgene approaches are expected to tolerate substantial rates of failure. Thus, self-elimination technology may permit rigorous field-based testing of gene drives by establishing strict time limits on the existence of gene drive transgenes in nature, rendering them essentially biodegradable. This article is part of the theme issue ‘Novel control strategies for mosquito-borne diseases'.
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DOI：
10.1126/science.aaa5945
发表时间：
2015-04-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gantz VM;Bier E
通讯作者：
Bier E
影响因子：
64.5
作者：
Hsu PD;Lander ES;Zhang F
通讯作者：
Zhang F
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
DOI：
10.1073/pnas.1713825115
发表时间：
2018-06-12
影响因子：
11.1
作者：
KaramiNejadRanjbar, Mohammad;Eckermann, Kolja N.;Wimmer, Ernst A.
通讯作者：
Wimmer, Ernst A.
DOI：
10.1002/bies.201500102
发表时间：
2016-01
期刊：
BioEssays : news and reviews in molecular, cellular and developmental biology
影响因子：
--
作者：
Gantz VM;Bier E
通讯作者：
Bier E