Electrochemical reaction of fructose dehydrogenase on carbon cryogel electrodes with controlled pore sizes
Electrochemical reaction of fructose dehydrogenase on carbon cryogel electrodes with controlled pore sizes
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DOI：
10.1016/j.elecom.2010.01.016
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发表时间：
2010-03
影响因子：
5.4
通讯作者：
S. Tsujimura;Akiko Nishina;Y. Hamano;K. Kano;S. Shiraishi
中科院分区：
文献类型：
--
作者：
S. Tsujimura;Akiko Nishina;Y. Hamano;K. Kano;S. Shiraishi
制备了孔径可控的碳冻凝胶（CCG）用于酶电极。以间苯二酚和甲醛为原料，CO 32-为催化剂，采用溶胶-凝胶法制备了CCG。通过改变间苯二酚与催化剂的摩尔比，可以控制平均孔径在5 ~ 40 nm之间。吸附在CCG电极上的果糖脱氢酶对果糖的氧化表现出很大的催化电流。催化电流的大小与孔径有关。当CCG的孔径小于FDH的孔径时，催化电流密度很低。另一方面，在实验条件下，观察到更宽的孔导致更高的催化电流密度。据推测，嵌入在碳介孔中的笼状酶易于与电极直接通信。
Carbon cryogels (CCG) with controlled porous size were developed for enzyme electrode. CCGs were prepared by sol–gel polycondensation of resorcinol and formaldehyde using CO32-as a catalyst. The average pore radius range from 5 to 40nm was controlled by changing the mole ratio of resorcinol to the catalyst. Fructose dehydrogenase adsorbed on a CCG electrode exhibited large catalytic currents for fructose oxidation without redox mediators. The catalytic current depended on the pore size. The catalytic current density was quite low when the pore size of CCG was small compared with the size of FDH. On the other hand, the wider pore resulted in higher catalytic current density was observed under the experimental conditions. It is assumed that caged enzymes embedded in the carbon mesopore are apt to communicate with the electrode directly.