PTHrP and PTH/PTHrP receptor expressions in human endometrium
PTHrP and PTH/PTHrP receptor expressions in human endometrium
复制标题
DOI：
10.1507/endocrj.48.219
复制
发表时间：
2001-04-01
影响因子：
2
通讯作者：
Okai, T
中科院分区：
文献类型：
--
作者：
Hoshi, S;Morimoto, T;Okai, T
我们研究月经周期依赖性的变化，PTHrP和PTH/PTHrP受体的表达在人子宫内膜免疫组化，竞争性逆转录聚合酶链反应（RT-PCR）。人子宫内膜组织获自因宫颈癌（原位癌）或卵巢癌而接受妇科手术的患者。20例患者的平均年龄为36.5岁（范围31-44）。对于mRNA表达的分析，使用来自增殖期（中期，n=5;晚期，n=5）和分泌期（早期，n=4;中期，n=4）的标本。免疫组化结果显示，PTHrP和PTH/PTHrP受体在上皮细胞和间质细胞的胞浆中均有表达。PTHrP在腺上皮细胞中的表达强于间质细胞。增殖期的染色强于分泌期，当比较来自同一患者的样品时，差异尤其显著。PTH/PTHrP受体也存在于子宫内膜的上皮细胞和基质细胞中。然而，在增殖期和分泌期之间没有观察到受体表达的差异。竞争性RT-PCR结果显示，增殖期PTHrP mRNA的表达高于分泌期，而PTH/PTHrP受体mRNA的表达无明显差异。这些数据表明，子宫内膜增殖可能是由局部PTHrP自分泌和/或旁分泌机制介导的。
We investigated menstrual cycle-dependent changes in the expression of PTHrP and PTH/PTHrP receptor in the human endometrium by immunohistochemistry, and competitive reverse transcription and polymerase chain reaction (RT-PCR). Human endometrial tissues were obtained from patients who underwent gynecological surgery due to cervical cancer (carcinoma in situ) or ovarian cancer. The mean age of the 20 patients was 36.5 (range 31-44) years. For analysis of mRNA expression, specimens from proliferative (mid, n=5; late, n=5) and secretory (early, n=4; mid, n=4) phases were used. Immunohistochemical expression of PTHrP and PTH/PTHrP receptor was observed in the cytoplasm of both epithelial and stromal cells. Stronger staining of PTHrP was found in glandular epithelial cells than in stromal cells. The staining during the proliferative phase was stronger than that in the secretory phase and the difference was particularly remarkable when comparing samples from the same patient. PTH/PTHrP receptor was also present in both epithelial and stromal cells of the endometrium. However, no difference was observed in receptor expression between the proliferative and secretory phases. Competitive RT-PCR revealed that the expression of PTHrP mRNA was higher during the proliferative phase than in the secretory phase, although no difference was observed in PTH/PTHrP receptor mRNA expression. The data suggest that endometrial proliferation may be mediated by a local PTHrP autocrine and/or paracrine mechanism.