Alterations of protein complexes and pathways in genetic information flow and response to stimulus contribute to Escherichia coli resistance to balofloxacin
Alterations of protein complexes and pathways in genetic information flow and response to stimulus contribute to Escherichia coli resistance to balofloxacin
复制标题
遗传信息流中蛋白质复合物和途径的改变以及对刺激的反应有助于大肠杆菌对巴洛沙星的耐药性
DOI：
10.1039/c2mb25090j
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Peng, Xuan-Xian
中科院分区：
文献类型：
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作者：
Li, Hui;Pan, Jian-Yi;Peng, Xuan-Xian
蛋白质-蛋白质相互作用是重要的生物过程，对全球了解细胞功能至关重要。到目前为止，人们对蛋白质的相互作用以及蛋白质相互作用网络和蛋白质复合体在细菌耐药性中的作用知之甚少。在本研究中，我们研究了大肠杆菌中暴露于抗生素巴洛沙星(BLFX)的蛋白质复合体。检测到1个与BLFX抗性有关的同源蛋白复合体和8个与BLFX抗性有关的异体蛋白复合体。这些复合体在BLFX抗性中的潜在作用被表征并归类为四个功能区：信息流、单糖代谢、对刺激的反应和氨基酸代谢过程。参与信息流和对刺激反应的蛋白质复合体在抗性中起着更重要的作用。这些结果与以前发表的通过gyrA-gyrB复合体获得喹诺酮类药物耐药的机制是一致的，并发现了两条新的抗生素耐药途径：遗传信息流上调和对刺激的反应改变。这两条途径的平衡将是降低BLFX抗性的可行方法。
Protein-protein interactions are important biological processes and essential for a global understanding of cell functions. To date, little is known about the protein interactions and roles of the protein interacting networks and protein complexes in bacterial resistance to antibiotics. In the present study, we investigated protein complexes in Escherichia coli exposed to an antibiotic balofloxacin (BLFX). One homomeric and eight heteromeric protein complexes involved in BLFX resistance were detected. Potential roles of these complexes that are played in BLFX resistance were characterized and categorized into four functional areas: information streams, monosaccharide metabolism, response to stimulus and amino acid metabolic processes. Protein complexes involved in information streams and response to stimulus played more significant roles in the resistance. These results are consistent with previously published mechanisms on the acquired quinolone-resistance through the GyrA-GyrB complex, and two novel antibiotic-resistant pathways were identified: upregulation of genetic information flow and alteration of the response to a stimulus. The balance of the two pathways will be a viable means of reducing BLFX-resistance.