Adrenomedullin regulates the speed of oviductal fluid flow in cattle
Adrenomedullin regulates the speed of oviductal fluid flow in cattle
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肾上腺髓质素调节牛输卵管液流的速度
DOI：
10.1002/mrd.22852
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发表时间：
2017
影响因子：
2.5
通讯作者：
Kimura K
中科院分区：
文献类型：
--
作者：
Yoshimoto Y;Nishie T;Ito S;Kobayashi Y;Yamamoto Y;Kiyoshi Okuda;Kimura K
输卵管液体从卵巢到壶腹子宫侧的单向流动在成功妊娠中起着重要作用，它是由纤毛跳动产生的。不同的系统调节纤毛跳动，如旁分泌、自分泌和内分泌。我们推测，肾上腺髓质素(ADM)是一种通过其受体发挥作用的多肽激素，它是降钙素受体样受体(CRLR)和受体活性修饰蛋白(RAMP)2或3的复合体，促进牛输卵管壶腹部的输卵管液体流动。首先，我们检测了ADM、CRLR、RAMP2和RAMP3mRNAs在动情周期中的表达，以及ADM受体蛋白成分在壶腹中的定位。此外，RAMP2蛋白仅在纤毛细胞中检测到，而CRLR和RAMP3蛋白在所有上皮细胞中均可检测到。用壶腹组织微球观察ADM和ADM拮抗剂对液体流速的影响。ADM拮抗剂降低珠粒转运速度，并被ADM逆转。此外，ADM还恢复了在无钙情况下降低的珠粒运输速度。总体而言，我们的结果提示ADM有助于壶腹输卵管液体流动的调节。
Unidirectional flow of oviductal fluid from the ovarian to uterine side of the ampulla plays a significant role in successful pregnancy, and is produced by ciliary beating. Various systems regulate ciliary beating, such as paracrine, autocrine, and endocrine. We hypothesized that Adrenomedullin (ADM)—a peptide hormone that acts via its receptors, which are complexes of Calcitonin receptor‐like receptor (CRLR) and Receptor activity‐modifying protein (RAMP) 2 or 3 — promotes oviductal fluid flow in the ampulla of bovine oviducts. First, we examined the expression ofADM,CRLR,RAMP2, andRAMP3mRNAs in isolated epithelial cells throughout the estrous cycle, and the localization of ADM receptor protein constituents in the ampulla.RAMP2expression was significantly higher in the follicular phase. Furthermore, RAMP2 protein was detected only in ciliated cells, whereas CRLR and RAMP3 were detected in all epithelial cells. The effects of ADM and an ADM antagonist on fluid‐flow speed were examined using microbeads in ampullary tissue. ADM antagonist decreased bead transport speed, and this decrease was reversed by ADM. In addition, ADM recovered the bead transport speed that decreased in the absence of calcium. Overall, our results suggest that ADM contributes to the regulation of oviductal fluid flow in ampulla.