Cellular and Cytokine Responses in the Granulomas of Asymptomatic Cattle Naturally Infected with Mycobacterium bovis in Ethiopia
Cellular and Cytokine Responses in the Granulomas of Asymptomatic Cattle Naturally Infected with Mycobacterium bovis in Ethiopia
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DOI：
10.1128/iai.00507-20
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发表时间：
2020-12-01
影响因子：
3.1
通讯作者：
Ameni, Gobena
中科院分区：
文献类型：
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作者：
Tulu, Begna;Martineau, Henny M.;Ameni, Gobena
在自然感染牛分枝杆菌的牛的淋巴结和组织中评估了细胞（CD3（+）T细胞和CD68（+）巨噬细胞）、细胞因子（干扰素γ阳性[IFN-γ（+）]和肿瘤坏死因子α阳性[TNF-α（+）]）和效应分子（诱导型一氧化氮合酶阳性[iNOS（+）]）反应。对 16 头奶牛进行了详细的尸检和免疫组织化学检查，这些奶牛通过单次皮内宫颈比较结核菌素 (SICCT) 测试呈阳性，并且是从埃塞俄比亚亚的斯亚贝巴市周围的奶牛场鉴定出来的。牛分枝杆菌培养阳性奶牛 (n = 12) 的肉眼病变严重程度显着高于培养阴性奶牛 (n = 4) (n = 4)。免疫组织化学技术显示，在培养阳性的奶牛中，随着肉芽肿阶段从I期增加到IV期，CD3(+)T细胞的平均免疫标记分数降低(P < 0.001)。相反，CD68(+)巨噬细胞、IFN-γ(+)、TNF-α(+)和iNOS(+)免疫标记分数从I期到IV期增加(P < 0.001)。在早期阶段，培养阴性奶牛的 CD68(+) 巨噬细胞 (P = 0.03) 和 iNOS(+) (P = 0.007) 免疫标记分数明显高于培养阳性奶牛。同样，在肉芽肿晚期阶段，培养阴性奶牛的 CD3(+) T 细胞平均比例显着高于培养阳性奶牛 (P , 0.001)。因此，本研究表明，奶牛自然感染牛支原体后，随着肉芽肿阶段从 I 期增加到 IV 期，CD3(+) 细胞的免疫标记分数减少，而 CD68(+) 巨噬细胞、IFN-gamma(+)、TNF-α(+) 和 iNOS(+) 免疫标记分数增加。
Cell (CD3(+) T cell and CD68(+) macrophages), cytokine (interferon gamma-positive [IFN-gamma(+)] and tumor necrosis factor alpha-positive [TNF-alpha(+)]), and effector molecule (inducible nitric oxide synthase-positive [iNOS(+)]) responses were evaluated in the lymph nodes and tissues of cattle naturally infected with Mycobacterium bovis. Detailed postmortem and immunohistochemical examinations of lesions were performed on 16 cows that were positive by the single intradermal cervical comparative tuberculin (SICCT) test and that were identified from dairy farms located around the city of Addis Ababa, Ethiopia. The severity of the gross lesion was significantly higher (P = 0.003) in M. bovis culture-positive cows (n = 12) than in culture-negative cows (n = 4). Immunohistochemical techniques showed that in culture-positive cows, the mean immunolabeling fraction of CD3(+) T cells decreased as the stage of granuloma increased from stage I to stage IV (P < 0.001). In contrast, the CD68(+) macrophage, IFN-gamma(+), TNF-alpha(+), and iNOS(+) immunolabeling fractions increased from stage I to stage IV (P < 0.001). In the early stages, culture-negative cows showed a significantly higher fraction of CD68(+) macrophage (P = 0.03) and iNOS(+) (P = 0.007) immunolabeling fractions than culture-positive cows. Similarly, at advanced granuloma stages, culture-negative cows demonstrated significantly higher mean proportions of CD3(+) T cells (P , 0.001) than culture-positive cows. Thus, this study demonstrates that, following natural infection of cows with M. bovis, as the stage of granuloma increases from stage I to stage IV, the immunolabeling fraction of CD3(+) cells decreases, while the CD68(+) macrophage, IFN-gamma(+), TNF-alpha(+), and iNOS(+) immunolabeling fractions increases.