Polymorphic microsatellite DNA markers from the oriental fruit fly Bactrocera dorsalis (Hendel)
Polymorphic microsatellite DNA markers from the oriental fruit fly Bactrocera dorsalis (Hendel)
复制标题
DOI：
10.1111/j.1471-8286.2004.00732.x
复制
发表时间：
2004-12-01
期刊：
影响因子：
--
通讯作者：
Chang, C
中科院分区：
文献类型：
--
作者：
Dai, SM;Lin, CC;Chang, C
从东方实蝇Bactrocera dorsalis（Hendel）中分离出6个多态性微卫星位点。为了评估它们作为高分辨率遗传标记的潜在效用，开发了聚合酶链反应（PCR）引物、扩增条件和自动荧光检测方案。在分析来自台湾六个不同地区的71个个体苍蝇，等位基因数从5到25不等的每个位点检测。这些位点的杂合度在0.268 ~ 0.737之间。这些微卫星标记在B群体结构和基因流研究中具有潜在的应用价值。dorsalis Mendel）。
Six polymorphic microsatellite loci are isolated from the Oriental fruit fly Bactrocera dorsalis (Hendel), an agricultural pest in Asia, including Taiwan. To assess their potential utility as high-resolution genetic markers, polymerase chain reaction (PCR) primers, amplification conditions, and an automated fluorescence detection protocol were developed. In analyses of 71 individual flies from six different areas of Taiwan, allele numbers ranged from five to 25 were detected for each locus. The observed heterozygosity ranged between 0.268 and 0.737 among these loci. No linkage disequilibrium was found. These microsatellite markers have potential utility to population structure and gene flow studies of B. dorsalis Mendel).