Genome-wide analysis of gene expression reveals gene regulatory networks that regulate chasmogamous and cleistogamous flowering in Pseudostellaria heterophylla (Caryophyllaceae).
Genome-wide analysis of gene expression reveals gene regulatory networks that regulate chasmogamous and cleistogamous flowering in Pseudostellaria heterophylla (Caryophyllaceae).
复制标题
全基因组基因表达分析揭示了调节太子参（石竹科）开花和闭锁开花的基因调控网络
DOI：
10.1186/s12864-016-2732-0
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发表时间：
2016-05-20
期刊：
影响因子：
4.4
通讯作者：
Song BH
中科院分区：
文献类型：
--
作者：
Luo Y;Hu JY;Li L;Luo YL;Wang PF;Song BH
背景太子参在同一个体上产生闭花（闭花受精，CL）和开放花（裂花受精，CH），但在不同季节。CH和CL花的产生可能是对环境变化的响应。为了更好地了解CH和CL开花的分子机制，我们比较了这两种类型的花的转录组，以研究差异基因表达模式，并确定控制CH和CL flowing.ResultsUsing RNA测序的基因调控网络，我们确定了同源的428个参与调控开花过程的拟南芥基因，并估计CH和CL花之间的差异基因表达模式。这些基因中的一些参与开花过程的基因调控网络表现出显着差异表达模式之间的CH和CL花。此外，我们还鉴定了CH和CL花之间另外396个差异表达的转录本。一些参与环境胁迫反应和类黄酮biosynthesis.ConclusionsWe提出如何差异表达的关键成员的三个基因调控模块可以解释CH和CL开花。未来的研究需要调查环境如何影响这些开花途径来调节CH和CL开花inP。异叶植物
BackgroundPseudostellaria heterophyllaproduces both closed (cleistogamous, CL) and open (chasmogamous, CH) flowers on the same individual but in different seasons. The production of CH and CL flowers might be in response to environmental changes. To better understand the molecular mechanisms of CH and CL flowering, we compared the transcriptome of the two types of flowers to examine differential gene expression patterns, and to identify gene regulatory networks that control CH and CL flowering.ResultsUsing RNA sequencing, we identified homologues of 428Arabidopsisgenes involved in regulating flowering processes and estimated the differential gene expression patterns between CH and CL flowers. Some of these genes involved in gene regulatory networks of flowering processes showed significantly differential expression patterns between CH and CL flowers. In addition, we identified another 396 differentially expressed transcripts between CH and CL flowers. Some are involved in environmental stress responses and flavonoid biosynthesis.ConclusionsWe propose how the differential expression of key members of three gene regulatory modules may explain CH and CL flowering. Future research is needed to investigate how the environment impinges on these flowering pathways to regulate CH and CL flowering inP. heterophylla.