Inhibitory effects of microalgae on the activation of hyaluronidase
Inhibitory effects of microalgae on the activation of hyaluronidase
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DOI：
10.1023/a:1012592620347
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发表时间：
2001-12-01
影响因子：
3.3
通讯作者：
Takenaka, H
中科院分区：
文献类型：
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作者：
Fujitani, N;Sakaki, S;Takenaka, H
研究了发菜、钝顶螺旋藻、紫菜、红景天、蛋白核小球藻、杜氏盐藻和卡氏侧耳等7种微藻对透明质酸酶活性的抑制作用。各微藻水提物的乙醇不溶部分的抑制作用强于乙醇可溶部分。钝顶螺旋藻、紫环沙门氏菌、海滨沙门氏菌、蛋白核沙门氏菌、盐生沙门氏菌和卡特雷沙门氏菌乙醇不溶部位的半数抑制浓度(IC50)分别为0.15、0.18、0.26、0.94、0.15和0.41 mg·L~(-1)。鞭毛藻的IC50没有被计算出来，因为没有检测到该藻的抑制作用。色甘酸二钠(DSCG)作为抗变态反应药物的IC50为0.14 mg mL(-1)。钝顶螺旋藻、紫色螺旋藻和盐藻的IC50与DSCG的IC50基本相同。这表明钝顶螺旋藻、紫色螺旋藻和盐藻的乙醇不溶部分可能是一种抗过敏物质。钝顶螺旋藻和盐藻的乙醇不溶部分通过分子排斥极限为20 kDa的膜进行超滤。残渣的IC50大于滤液的IC50。这些结果表明，这些微藻的抗过敏物质(S)可能是多糖。
The inhibitory effects of seven microalgae, Nostoc flagelliforme, Spirulina platensis, Porphyridium purpureum, Rhodosorus marinus, Chlorella pyrenoidosa, Dunaliella salina and Pleurochrysis carterae on the activation of hyaluronidase were evaluated. The inhibitory effect of the ethanol-insoluble fraction of each water extract from microalgae was stronger than that of the ethanol-soluble fraction. The 50% inhibitory concentration (IC50) of the ethanol-insoluble fraction of S. platensis, P. purpureum, R. marinus, C. pyrenoidosa, D. salina and P. carterae was 0.15, 0.18, 0.26, 0.94, 0.15 and 0.41 mg mL(-1), respectively. The IC50 of N .flagelliforme was not calculated, because there was no detectable inhibitory effect of this alga. The IC50 of disodium cromoglycate (DSCG) used as the anti-allergic medicine was 0.14 mg mL(-1). The IC50 of S. platensis, P. purpureum and D. salina were almost the same as that of DSCG. This suggests that the ethanol-insoluble fraction of S. platensis, P. purpureum and D. salina might be an anti-allergic substance. The ethanol-insoluble fraction of S. platensis and D. salina was ultrafiltered through a membrane having a molecular exclusion limit of 20 kDa. The IC50 of the residue was stronger than that of the filtrate. These results suggest that the anti-allergic substance(s) of these microalgae may be polysaccharides.