Apparent richness and community composition of Bacteria and Archaea in geothermal springs
Apparent richness and community composition of Bacteria and Archaea in geothermal springs
复制标题
DOI：
10.3354/ame01333
复制
发表时间：
2009-01-01
影响因子：
1.4
通讯作者：
Kamagata, Yoichi
中科院分区：
文献类型：
--
作者：
Kormas, Konstantinos Ar.;Tamaki, Hideyuki;Kamagata, Yoichi
研究了希腊Polihnitos（89 ℃）、Edipsos（81.8 ℃）、Thermopiles（38.9 ℃）、Eleftheres（41.2 ℃）和Lagadas（35.2 ℃）地热泉的古细菌和细菌16 S rRNA基因差异。原核细胞的丰度在0.02 × 10 ~（-5）~ 0.92 × 10 ~（-5）cells ml ~（-1）之间。共分析了227个古细菌和501个细菌克隆，分别归属于85个和121个操作分类单位（OTU）。库克隆覆盖率，根据良好的C估计，是令人满意的（>75%），除了在Thermopiles（类似于40%）和Eleftheres（类似于60%）的古菌的古菌类型大多数有关的序列尚未培养的微生物从陆地地热泉，深海热液喷口和地下。一个更高的数量的细菌菌落类型与类似环境中培养的微生物有关。大多数已发现的细菌类型的嗜热性也得到了它们高G+C含量的支持，这与泉水的温度呈正相关。泉水中细菌和细菌的多样性表现出不同的模式，细菌仅在Polihnitos和Lagadas泉水中具有较高的多样性。Shannon多样性指数H'的变化范围为：浮游植物（0.23 ~ 3.44），细菌（1.22 ~ 3.03），与温度、pH、盐度和溶解氧含量无关。细菌克隆文库和细菌克隆文库分别含有50%~ 94.1%和68.8%~ 96.2%的稀有菌型（即在克隆文库中只出现一次或两次的菌型），表明稀有菌型在形成群落多样性中的重要性。
The archaeal and bacterial 16S rRNA gene diversities of the Polihnitos (89 degrees C), Edipsos (81.8 degrees C), Thermopiles (38.9 degrees C), Eleftheres (41.2 degrees C) and Lagadas (35.2 degrees C) geothermal springs in Greece were investigated. The abundance of prokaryotic cells varied between 0.02 x 10(-5) and 0.92 x 10(-5) cells ml(-1). A total of 227 archaeal and 501 bacterial clones were analysed, which were attributed to 85 and 121 operational taxonomic units (OTUs), respectively. Library clone coverage, based on Good's C estimator, was satisfactory (>75%), except for the Archaea in Thermopiles (similar to 40%) and Eleftheres (similar to 60%) Most of the archaeal phylotypes were related to sequences of yet-uncultivated microorganisms retrieved from terrestrial geothermal springs, deep-sea hydrothermal vents and the Subsurface. A much higher number of bacterial phylotypes was related to cultivated microorganisms from similar environments. The thermophilic nature of most of the discovered phylotypes was also Supported by their high G+C content, which was positively correlated with the springs' tempera tu res. The springs Showed different diversity patterns for Bacteria and Archaea, with Bacteria having higher diversity only in Polihnitos and Lagadas springs. The Shannon diversity index H' showed larger variation for Archaea (0.23 to 3.44) than for Bacteria (1.22 to 3.03) and was unrelated to the prevailing temperature, pH, salinity and dissolved oxygen content. Archaeal and bacterial clone libraries respectively contained 50 to 94.1 and 68.8 to 96.2% rare phylotypes (i.e. those that appear only once or twice in the clone library), indicating the importance of rare phylotypes in shaping community diversity.