Genetic Manipulations of Oral Spirochete Treponema denticola.
Genetic Manipulations of Oral Spirochete Treponema denticola.
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DOI：
10.1007/978-1-0716-0939-2_2
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Li C
中科院分区：
文献类型：
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作者：
Kurniyati K;Li C
在口腔中已经鉴定出60多种不同的口腔密螺旋体，但只有少数几种可以在体外可靠地培养。在这些可培养的物种中，齿密螺旋体是与人类牙周炎相关的关键病原体之一，由于其医学价值和遗传易感性，已成为了解口腔密螺旋体物种遗传学、病原学和病理生理学的典范模式生物。在过去的二十年里，已经开发了几种遗传工具，这些工具在齿纹夜蛾的研究中发挥了重要作用。本章描述了齿纹夜蛾遗传操作的实验设计和程序。
There have been more than 60 different oral Treponema species identified in the oral cavity; however, only few species can be cultivated in vitro reliably. Among those cultivable species, due to its medical importance and genetic tractability, Treponema denticola, one of the keystone pathogens associated with human periodontitis, has emerged as a paradigm model organism to understanding the genetics, etiology, and pathophysiology of oral Treponema species. During the last two decades, several genetic tools have been developed, which have played an instrumental role in the study of T. denticola. This chapter describes the experimental design and procedure of genetic manipulations of T. denticola.