Metabolism of DHEA in postmenopausal women following percutaneous administration
Metabolism of DHEA in postmenopausal women following percutaneous administration
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DOI：
10.1016/j.jsbmb.2006.09.034
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发表时间：
2007-02-01
影响因子：
4.1
通讯作者：
Leclaire, Jacques
中科院分区：
文献类型：
--
作者：
Labrie, Fernand;Belanger, Alain;Leclaire, Jacques
血清脱氢表雄酮（DHEA）随年龄的显著下降被认为在与衰老相关的健康问题中起作用，这些健康问题通过外源性施用DHEA可能是可预防的或可逆的。在本研究中，液相色谱/质谱/质谱使用LC/MS/MS和气相色谱/质谱法（GC/MS）测量75名60-65岁的白人妇女的DHEA及其代谢物II的血清水平，这些妇女接受3g 0.1%、0.3%、1.0%或2.0%DHEA乳膏或安慰剂，每天两次施用在面部、上胸部、胳膊和腿在2.0%DHEA剂量下，DHEA的血清水平比对照组增加574%，而雄激素代谢物雄甾酮葡糖苷酸（ADT-G）、3 α-雄烯二醇-3G（3 α-二醇-3G）和3 α-二醇-17 G的总和仅增加231%。另一方面，血清睾酮和二氢甾酮分别增加了192%和275%，高于基础水平，而雌酮和雌二醇分别为139%和158%。这些数据表明，外源性DHEA在绝经后妇女中的转化优先转化为雄激素而不是雌激素。另一方面，目前的数据表明，在绝经后妇女补充DHEA后的血清DHEA测量是活性雄激素和雌激素的形成的高估，并表明随着年龄的增长，DHEA转化为雄激素和雌激素的效率降低。(c)2006爱思唯尔有限公司保留所有权利。
The marked decline in serum dehydroepiandrosterone (DHEA) with age is believed to play a role in health problems associated with aging, these health issues being potentially preventable or reversible by the exogenous administration of DHEA. In the present study, liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) and gas chromatrography/mass spectrometry (GC/MS) were used to measure the serum levels of DHEA and I I of its metabolites in seventy-five 60-65-year-old Caucasian women who received 3 g of 0.1%, 0.3%, 1.0% or 2.0% DHEA cream or placebo applied twice daily on the face, upper chest, arms and legs. The serum levels of DHEA increased 574% over control at the 2.0% DHEA dose while the sum of the androgen metabolites androsterone glucuronide (ADT-G), 3 alpha-androstenediol-3G (3 alpha-diol-3G) and 3 alpha-diol-17G increased by only 231%. On the other hand, serum testosterone and dihydrosterone were increased by 192% and 275%, respectively, above basal levels compared to 139% and 158% for estrone and estradiol. Such data show that the transformation of exogenous DHEA in postmenopausal women is preferentially into androgens rather than into estrogens. On the other hand, the present data indicate that serum DHEA measurements following DHEA supplementation in postmenopausal women are an overestimate of the formation of active androgens and estrogens and suggest a decreased efficiency of transformation of DHEA into androgens and estrogens with aging. (c) 2006 Elsevier Ltd. All rights reserved.