Oral immunization of swine with attenuated Salmonella typhimurium aroA SL3261 expressing a recombinant antigen of Mycoplasma hyopneumoniae (NrdF) primes the immune system for a NrdF specific secretory IgA response in the lungs
Oral immunization of swine with attenuated Salmonella typhimurium aroA SL3261 expressing a recombinant antigen of Mycoplasma hyopneumoniae (NrdF) primes the immune system for a NrdF specific secretory IgA response in the lungs
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DOI：
10.1006/mpat.2000.0412
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发表时间：
2001-02-01
影响因子：
3.8
通讯作者：
Djordjevic, SP
中科院分区：
文献类型：
--
作者：
Fagan, PK;Walker, MJ;Djordjevic, SP
用表达重组肺炎支原体抗原的鼠伤寒沙门菌SL3261 （aroA突变体）口服免疫猪流行性肺炎。该构建体命名为S. typhimurium aroA SL3261 (pKF1)，表达了一个含有42 kDa的肺炎支原体NrdF核糖核苷酸还原酶R2亚基蛋白羧基末端11 kDa的重组蛋白。在这里，我们证明该抗原存在于所有七种地理上不同的猪肺炎支原体菌株中，并且在实验感染强毒性猪肺炎支原体博福特菌株后被猪免疫系统识别。用鼠伤寒沙门氏菌SL3261 （pKF1）口服免疫猪第0天和第14天的免疫应答进行评价。口服鼠伤寒沙门氏菌SL3261 （pKF1）免疫启动免疫系统，引起显著的(P
Salmonella typhimurium SL3261 (aroA mutant) expressing a recombinant Mycoplasma hyopneumoniae antigen was used to orally immunize swine against porcine enzootic pneumonia. This construct, designated S. typhimurium aroA SL3261 (pKF1), expressed a recombinant protein containing the carboxy-terminal 11 kDa of a 42 kDa M. hyopneumoniae NrdF ribonucleotide reductase R2 subunit protein. Here we demonstrate that this antigen is present in all seven geographically diverse strains of M. hyopneumoniae tested, and is recognized by the swine immune system after experimental infection with the virulent M. hyopneumoniae Beaufort strain. The immune response of swine orally immunized twice with S. typhimurium SL3261 (pKF1) on day 0 and day 14 was evaluated. Oral immunization with S. typhimurium SL3261 (pKF1) primed the immune system to elicit a significant (P