A protein-based approach to mark arthropods for mark-capture type research
A protein-based approach to mark arthropods for mark-capture type research
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DOI：
10.1111/j.1570-7458.2010.00980.x
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发表时间：
2010-05-01
影响因子：
1.9
通讯作者：
Jones, Vincent P.
中科院分区：
文献类型：
--
作者：
Hagler, James R.;Jones, Vincent P.
进行了一系列的研究，以测试方法标记各种各样的节肢动物与廉价的蛋白质标记捕获扩散研究。检测的标志物包括鸡蛋清中的卵白蛋白和牛奶中的酪蛋白。第一项研究通过两个应用程序验证了这两种标记对50多种棉花节肢动物的有效性。施用方法包括：（1）局部加残留蛋白质施用，和（2）仅残留蛋白质施用。这两种蛋白质标记，无论应用的方法，很容易保留在节肢动物组合的研究期间。第二项研究确定了昆虫在接触喷洒有蛋清溶液的棉花组织后获得鸡卵清蛋白的速度。在实验室条件下，绝大多数成虫Hippodamia convergens Guerin-Meneville（鞘翅目：瓢虫科）和草盲蝽Lygus hesperus Knight（异翅目：盲蝽科）在5 min后获得标记，未成熟的粉纹夜蛾Trichoplusia ni（Hubner）（鳞翅目：夜蛾科）在40 min后获得标记。convergens和L.通过接触苜蓿、紫花苜蓿、紫花苜蓿、（豆科），或莱斯克雷拉属（lesquerella），莱斯克雷拉·芬德勒里（Lesquerella fendleri）（沃森）（豆科），用牛乳溶液喷洒的植物。这些昆虫在田间条件下迅速从植物残留物中获得酪蛋白标记。最后一项研究确定了H。Convergens在暴露于含有7天的牛乳残留物的苜蓿和Lesquerella植物24小时后保留酪蛋白。约95%的H. Convergens在从每种植物中取出后保持酪蛋白标记2天。
A series of studies was conducted to test methods for marking a wide variety of arthropods with inexpensive proteins for mark-capture dispersal research. The markers tested included egg albumin protein in chicken egg whites and casein protein in bovine milk. The first study qualified the effectiveness of the two marks on more than 50 arthropod species inhabiting cotton via two application procedures. The application methods included: (1) a topical plus residue protein application, and (2) a residue-only protein application. Both protein marks, regardless of the method of application, were readily retained on the arthropod assemblage over the duration of the study. The second study determined how rapidly insects acquire chicken egg albumin protein after contact exposure to cotton tissue sprayed with an egg whites solution. Under laboratory conditions, the vast majority of adult Hippodamia convergens Guerin-Meneville (Coleoptera: Coccinellidae) and Lygus hesperus Knight (Heteroptera: Miridae) acquired the mark after 5 min, and immature Trichoplusia ni (Hubner) (Lepidoptera: Noctuidae) acquired the marker after 40 min. The third study determined how rapidly H. convergens and L. hesperus acquire bovine casein protein after contact exposure to either alfalfa, Medicago sativa L. (Fabaceae), or lesquerella, Lesquerella fendleri (Watson) (Brassicaceae), plants sprayed with a bovine milk solution. These insects rapidly acquired the casein mark from the plant residue under field conditions. A final study determined how long H. convergens retain casein protein after 24-h exposure to alfalfa and lesquerella plants containing a 7-day-old residue of bovine milk. Approximately 95% of the H. convergens maintained the casein mark for 2 days after removal from each type of plant.