Dantrolene reduces platelet-derived growth factor (PDGF)-induced vascular smooth muscle cell proliferation and neointimal formation following vascular injury in mice
Dantrolene reduces platelet-derived growth factor (PDGF)-induced vascular smooth muscle cell proliferation and neointimal formation following vascular injury in mice
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丹曲林可减少小鼠血管损伤后血小板衍生生长因子 (PDGF) 诱导的血管平滑肌细胞增殖和新内膜形成
DOI：
10.1016/j.bbrc.2022.07.038
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发表时间：
2022
影响因子：
3.1
通讯作者：
Yano Masafumi
中科院分区：
文献类型：
--
作者：
Sakai Chihiro;Mikawa Mei;Yamamoto Takeshi;Uchida Tomoyuki;Nakamura Yoshihide;Akase Hideaki;Suetomi Takeshi;Tominaga Naoomi;Inamitsu Masako;Oda Tetsuro;Okamura Takayuki;Kobayashi Shigeki;Yano Masafumi
丹曲林是一种兰尼定受体阻滞剂，临床上用于治疗恶性高热。本研究采用小鼠主动脉血管平滑肌细胞(MOVAS)和小鼠动脉损伤模型，观察丹曲林对血管平滑肌细胞增殖和迁移的抑制作用，探讨丹曲林是否能抑制血小板衍生生长因子(PDGF)诱导的血管平滑肌细胞增殖和迁移。用免疫组织化学方法检测丹曲林对血管平滑肌表型的影响。此外，使用小鼠动脉损伤模型，通过在血管周围应用丹曲林来测试平滑肌细胞的增殖和表型转换。丹曲林可抑制PDGF诱导的细胞增殖和细胞迁移。丹曲林还能抑制血管收缩表型向合成表型的转变。丹曲林能有效地抑制小鼠动脉损伤后血管平滑肌细胞的增殖、迁移和新生内膜形成。
Dantrolene is a ryanodine receptor blocker that is used clinically for treatment of malignant hyperthermia. This study was conducted using murine aortic vascular smooth muscle cells (MOVAS) and a mouse arterial injury model to investigate the inhibitory effect of dantrolene on smooth muscle cell proliferation and migration.We investigated whether dantrolene suppressed platelet-derived growth factor (PDGF)-induced vascular smooth muscle cell proliferation and migrationin vitro. The effect of dantrolene on smooth muscle phenotype was evaluated using immunostaining. In addition, smooth muscle cell proliferation and phenotype switching were tested by applying dantrolene around blood vessels using a mouse arterial injury model. Dantrolene inhibited PDGF-induced cell proliferation and migration of MOVAS. Dantrolene also inhibited the switch from contractile to synthetic phenotype bothin vitroandin vivo.Dantrolene is effective at inhibiting vascular smooth muscle cell proliferation, migration, and neointimal formation following arterial injury in mice.