Dielectrophoretic micropatterning with microparticle monolayers covalently linked to glass surfaces
Dielectrophoretic micropatterning with microparticle monolayers covalently linked to glass surfaces
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DOI：
10.1021/la048111p
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发表时间：
2004-12-07
期刊：
影响因子：
3.9
通讯作者：
Matsue, T
中科院分区：
文献类型：
--
作者：
Suzuki, M;Yasukawa, T;Matsue, T
利用负介电电泳力在玻璃基底上制备二维微图案，图案化的微颗粒通过交联剂共价结合在基底上。利用负介电电泳（n-DEP）的排斥力制备了线状和网格状的微粒。将模板叉指型微带阵列（IDA）电极（宽度和间隙50 μ m）并入具有流体通道的介电泳图案化单元中。以辛二酸二琥珀酰亚胺酯（DSS）和间马来酰亚胺苯甲酰-N-羟基琥珀酰亚胺酯（MBS）为交联剂，将带有氨基或巯基的微结构固定在玻璃基片上。黄递酶（Dp），一种黄素酶，选择性地附着在图案化的微粒使用马来酰亚胺基团的MBS。在NADH和二茂铁甲醇作为氧化还原介体的存在下，用扫描电化学显微镜（SECM）对图案化颗粒上的酶活性进行电化学表征。SECM图像证明Dp被选择性地固定在微粒表面以保持其催化活性。
Two-dimensional micropatterns of microparticles were fabricated on glass substrates with negative dielectrophoretic force, and the patterned microparticles were covalently bound on the substrate via cross-linking agents. The line and grid patterns of microparticles were prepared using the repulsive force of negative dielectrophoresis (n-DEP). The template interdigitated microband array (IDA) electrodes (width and gap 50 mum) were incorporated into the dielectrophoretic patterning cell with a fluidic channel. The microstructures on the glass substrates with amino or sulfhydryl groups were immobilized with the cross-linking agents disuccinimidyl suberate (DSS) and m-maleimidobenzoyl-N-hydroxy-succinimide ester (MBS). Diaphorase (Dp), a flavoenzyme, was selectively attached on the patterned microparticles using the maleimide groups of MBS. The enzyme activity on the patterned particles was electrochemically characterized with a scanning electrochemical microscope (SECM) in the presence of NADH and ferrocenylmethanol as a redox mediator. The SECM images proved that Dp was selectively immobilized onto the surface of microparticles to maintain its catalytic activity.