Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching
Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching
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受磷蛋白基因的转录调控和 SERCA2 基因的翻译调控在骨骼肌表型转换过程中产生这两种肌浆网蛋白的协调表达
DOI：
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发表时间：
1995
影响因子：
4.8
通讯作者：
F. Briggs
中科院分区：
文献类型：
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作者：
P. Hu;C. Yin;K.;L. D. Wright;Todd E. Nixon;Andrew S. Wechsler;John A. Spratt;F. Briggs
慢性1赫兹刺激犬背阔肌产生一种时间依赖性的从快抽动到慢抽动的转换。这包括肌浆网蛋白质的变化。肌肉刺激3d后，快抽动Ca-ATPase(SERCA1a)基因表达下调，慢抽动Ca-ATPase(SERCA2a)基因表达下调；刺激14d后，两种基因表达的变化基本完成。虽然磷蛋白mRNA的诱导在肌肉刺激3d后就开始了，但其上调直到肌肉刺激42d才完成。SERCA2a蛋白和SERCA2a基因表达的时程差异很大，提示SERCA2基因的表达既受转录水平的调控，又受翻译水平的调控。磷蛋白表达的时间进程与磷蛋白mRNA的表达过程密切相关，表明该基因受转录调控。因此，SERCA2a和磷蛋白蛋白的协调表达是通过SERCA2基因的翻译控制和磷蛋白基因的转录控制实现的。
Chronic 1 Hz stimulation of the canine latissimus dorsi muscle produced a time-dependent switch from the fast-twitch to the slow-twitch phenotype. This included changes in the proteins of the sarcoplasmic reticulum. After 3 days of muscle stimulation, there was down- regulation of fast-twitch Ca-ATPase (SERCA1a) mRNA and induction of slow-twitch Ca-ATPase (SERCA2a) mRNA; most changes in both mRNAs were nearly complete after 14 days of stimulation. Although the induction of phospholamban mRNA began after 3 days of muscle stimulation, its up- regulation was not completed until the muscle had been stimulated for 42 days. The time course of expression of SERCA2a protein was very different from that of SERCA2a mRNA, suggesting that SERCA2 gene expression is regulated at the translational as well as the transcriptional level. The time course of expression of phospholamban protein closely followed that of phospholamban mRNA, suggesting that this gene is under transcriptional control. Thus coordinated expression of SERCA2a and phospholamban proteins is achieved via translational control of the SERCA2 gene and transcriptional control of the phospholamban gene.