INHIBITION OF LIMB CHONDROGENESIS BY FIBRONECTIN
INHIBITION OF LIMB CHONDROGENESIS BY FIBRONECTIN
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DOI：
10.1111/j.1432-0436.1984.tb01371.x
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
SOLURSH, M
中科院分区：
文献类型：
--
作者：
SWALLA, BJ;SOLURSH, M
比较了高密度培养物的高密度培养物的高密度培养物，或比较了高密度培养物中的23/24 [鸡胚]肢体间质的阶段的近端区域。远端培养物经历了广泛的软骨生成，无论是否在F12培养基中补充了10％的胎牛血清，5％胎牛血清或纤连蛋白。近端培养物无法在含有10％胎牛血清或纤连蛋白的培养基中经历软骨，但在含有降低的血清浓度或无血清的培养基中确实形成软骨。如果细胞在天然I型胶原蛋白凝胶中的低密度下培养，则近端细胞在存在纤连蛋白的情况下具有降低的软骨生成能力，而远端细胞的软骨发生不受纤连蛋白的添加影响。近端和远端细胞对血清和纤连蛋白的反应差异。细胞对细胞外基质的普遍成分的反应可能会随发育而变化。
Chondrogenic capacity of high density cultures prepared from the developmentally younger, distal region or more advanced proximal region of stage 23/24 [chick embryo] limb mesenchyme in high density cultures was compared. Distal cultures undergo extensive chondrogenesis whether in F12 medium supplemented with 10% fetal calf serum, 5% fetal calf serum or fibronectin. Proximal cultures fail to undergo chondrogenesis in medium containing 10% fetal calf serum or fibronectin, but do form cartilage in medium containing a decreased serum concentration or no serum. If the cells are cultured at low densities in native type-I collagen gels, proximal cells have a reduced chondrogenic capacity in the presence of fibronectin, while chondrogenesis by distal cells is unaffected by the addition of fibronectin. Proximal and distal cells respond differentially to serum and to fibronectin. Response of the cell to prevalent components of the extracellular matrix might change with development.