Equine amniotic membrane transplantation for corneal ulceration and keratomalacia in three horses
Equine amniotic membrane transplantation for corneal ulceration and keratomalacia in three horses
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DOI：
10.1111/j.1463-5224.2005.00405.x
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发表时间：
2005-09-01
影响因子：
1.6
通讯作者：
Gelatt, KN
中科院分区：
文献类型：
--
作者：
Lassaline, ME;Brooks, DE;Gelatt, KN
目的羊膜具有抗纤维化、抗血管生成和抗蛋白酶活性。这项回顾性研究的目的是探讨使用马羊膜移植（AMT）在保留视力，保持结构完整的地球仪和最大限度地提高美观的马眼睛角膜溃疡和严重keratomalacia.Methods马羊膜先前已无菌收获从一个12岁的纯种母马在择期剖腹产手术。将羊膜切片储存在-80 ℃下，并根据需要解冻。我们对佛罗里达大学患有角膜软化症的马的病例记录进行了检查，这些病例接受了AMT而没有进行结膜移植。记录临床描述、内科和外科治疗详情、地球仪存活率和视力结果。通过细胞学、培养或组织学检查确定病因。结果三匹角膜溃疡和严重角膜软化症的马在2002年12月至2003年4月期间接受了无结膜移植的AMT。假单胞菌从所有三只眼睛中培养出来，有两只眼睛同时存在真菌感染的证据。三个溃疡分别为角膜直径的50%、72%和76%，在积极的药物治疗下，每个溃疡都恶化了。在所有三种情况下，AMT脱落超过4至6周。在最后一次随访时，接受AMT的所有三只眼睛都很舒适，没有接受药物治疗，有光感和不一致的位置依赖性威胁反应。所有三匹马返回到他们以前的works.Conclusions结果表明，少量的马AMT羊膜可以成功地用于保存地球仪的结构和有限的视力，以及优化美容，在马眼角膜溃疡和严重的角膜软化症。
Purpose Amniotic membrane has antifibrotic, anti-angiogenic and antiprotease properties. This retrospective study was designed to investigate the use of equine amniotic membrane transplants (AMT) at preserving vision, maintaining the structural integrity of the globe and maximizing cosmesis in equine eyes with corneal ulceration and severe keratomalacia.Methods Equine amnion had previously been aseptically harvested from a 12-year-old Thoroughbred mare during an elective Cesarean section. Sections of amnion were stored at -80 degrees C and thawed as needed. Records of equine cases at the University of Florida with keratomalacia that received an AMT without adjunctive conjunctival grafting were examined. Clinical description, details of medical and surgical treatment, globe survival and visual outcome were documented. Etiologies were determined by cytology, culture or histology.Results Three horses with corneal ulceration and severe keratomalacia received an AMT without conjunctival graft between December 2002 and April 2003. Pseudomonas spp. were cultured from all three eyes, with evidence of a concurrent fungal infection in two eyes. The three ulcers were 50, 72, and 76% of corneal diameter, and each one worsened in the face of aggressive medical therapy. In all three cases, the AMT sloughed over a 4 to 6-week period. At last follow-up, all three eyes receiving AMT were comfortable and receiving no medication, with light perception and an inconsistent location-dependent menace response. All three horses returned to their prior work.Conclusions Results of a small number of equine AMT suggest that amnion can be used successfully to preserve both globe structure and limited vision, as well as optimize cosmesis, in horse eyes with corneal ulceration and severe keratomalacia.