Smartphone-enabled optofluidic exosome diagnostic for concussion recovery.
Smartphone-enabled optofluidic exosome diagnostic for concussion recovery.
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DOI：
10.1038/srep31215
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发表时间：
2016-08-08
影响因子：
4.6
通讯作者：
Issadore D
中科院分区：
文献类型：
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作者：
Ko J;Hemphill MA;Gabrieli D;Wu L;Yelleswarapu V;Lawrence G;Pennycooke W;Singh A;Meaney DF;Issadore D
改善脑震荡治疗的一个主要障碍是我们目前无法识别受伤后将经历持续问题的患者。近年来，脑源性外切体通过血脑屏障在脑损伤后循环，作为脑功能恢复的非侵入性生物标志物显示出巨大的潜力。然而，由于其体积小(30-100 nm)和传统外切体测量所需的大量样品制备(>；24 小时)，外切体的临床应用一直受到限制。为了应对这些挑战，我们开发了一个支持智能手机的光流控平台来测量脑源性外切体。我们芯片上的样本到答案的时间是1 小时，比传统技术快10倍。关键的创新是一种光流控设备，可以检测酶放大的外切体生物标记物，并使用智能手机摄像头读取。使用这种方法，我们在体外和小鼠脑震荡模型中检测和分析了损伤后状态下的GluR2+外切体。
A major impediment to improving the treatment of concussion is our current inability to identify patients that will experience persistent problems after the injury. Recently, brain-derived exosomes, which cross the blood-brain barrier and circulate following injury, have shown great potential as a noninvasive biomarker of brain recovery. However, clinical use of exosomes has been constrained by their small size (30–100 nm) and the extensive sample preparation (>24 hr) needed for traditional exosome measurements. To address these challenges, we developed a smartphone-enabled optofluidic platform to measure brain-derived exosomes. Sample-to-answer on our chip is 1 hour, 10x faster than conventional techniques. The key innovation is an optofluidic device that can detect enzyme amplified exosome biomarkers, and is read out using a smartphone camera. Using this approach, we detected and profiled GluR2+ exosomes in the post-injury state using both in vitro and murine models of concussion.