Analysis of intra-uterine cytokine concentration and matrix-metalloproteinase activity in women with recurrent failed embryo transfer
Analysis of intra-uterine cytokine concentration and matrix-metalloproteinase activity in women with recurrent failed embryo transfer
复制标题
DOI：
10.1093/humrep/deg139
复制
发表时间：
2003-03-01
影响因子：
6.1
通讯作者：
Wilkinson, D
中科院分区：
文献类型：
--
作者：
Inagaki, N;Stern, C;Wilkinson, D
背景：在所有体外受精项目中，一些患者即使经过多次胚胎移植手术也未能实现持续妊娠。子宫内不利的环境可能是导致这种反复着床失败的一个因素。通过测量此类患者子宫冲洗液中的细胞因子浓度和基质金属蛋白酶活性来解决这个问题。方法和结果：22 名之前接受过至少 10 个胚胎移植但未持续妊娠的患者在黄体期进行了子宫腔冲洗。测定所得液体中白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ、白血病抑制因子(LIF)、IL-10和基质金属蛋白酶(MMP)-2和-9活性的浓度。将结果与已知有生育能力的对照女性群体 (n = 16) 以及反复自然流产的女性 (n = 13) 进行比较。反复着床失败组的MMP评分和IL-1β浓度显着高于对照组，而IFN-γ和IL-10浓度显着低于对照组。结论：在反复植入失败的 IVF 患者中，观察到子宫内细胞因子浓度和 MMP 活性的变化模式。
BACKGROUND: In all IVF programmes, some patients fail to achieve an ongoing pregnancy, even after numerous embryo transfer procedures. An unfavourable environment within the uterus might be a contributory factor to such recurrent implantation failure. This question was addressed by measuring cytokine concentrations and matrix metalloproteinase activities in fluid derived from uterine irrigation of such patients. METHODS AND RESULTS: The uterine cavities of 22 patients who had previously undergone embryo transfer of at least 10 embryos without ongoing pregnancy were irrigated during the luteal phase. The resultant fluid was assayed for the concentration of interleukin (IL)-1beta, tumour necrosis factor (TNF)-alpha, interferon (IFN)-gamma, leukaemia inhibitory factor (LIF), IL-10 and matrix metalloproteinase (MMP) -2 and -9 activity. The results were compared with those of a control population of women known to be previously fertile (n = 16) and also with women with recurrent spontaneous abortion (n = 13). In the recurrent implantation failure group, the MMP score and IL-1beta concentration were significantly higher than those in the control group, whereas concentrations of IFN-gamma and IL-10 were significantly lower. CONCLUSIONS: In IVF patients with recurrent implantation failure, an altered pattern of intra-uterine cytokine concentration and MMP activity was observed.