EFFECT OF SECRETORY PARTICLES IN BOVINE SEMINAL-VESICLE SECRETION ON SPERM MOTILITY AND ACROSOME REACTION
EFFECT OF SECRETORY PARTICLES IN BOVINE SEMINAL-VESICLE SECRETION ON SPERM MOTILITY AND ACROSOME REACTION
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DOI：
10.1530/jrf.0.0790409
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发表时间：
1987-03-01
期刊：
影响因子：
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通讯作者：
VANHAPERTTULA, T
中科院分区：
文献类型：
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作者：
AGRAWAL, Y;VANHAPERTTULA, T
牛精囊分泌物中发现的颗粒通过离心法进行浓缩。它们的大小和形态各不相同，具有镁离子、钙激活的ATPase、氨基肽酶A、丙氨酰氨基肽酶、γ-谷氨酰转肽酶和二肽基肽酶IV的活性。将这些颗粒悬浮在改良的Ringer培养液中，可引起精子的高度激活和顶体反应。通过显微镜玻片试验分析，这种过度激活在暴露于膜颗粒后的几分钟内开始，并持续3-4小时，在此期间精子经历了顶体反应。顶体染色、相差显微镜和透射电子显微镜显示，在37℃下，顶体反应在60min内开始，4h内影响高达80%的精子，这些膜颗粒在酶组成、功能和起源器官方面与以往报道的其他物种不同。
Particles found in bovine seminal vesicle secretion were enriched by centrifugation. They varied in size and morphology and contained Mg2+, Ca2+-activated ATPase, aminopeptidase A, alanyl aminopeptidase, .gamma.-glutamyl transpeptidase and dipeptidyl peptidase IV activities. Hyperactivation of sperm motility and the acrosome reaction were induced by these particles in epididymal spermatozoa suspended in a modified Ringer medium. The hyperactivation, analysed with a microscopic slide test, started within minutes of exposure to membrane particles and continued for 3-4 h, during which time spermatozoa underwent the acrosome reaction. Acrosome staining, phase-contrast microscopy and transmission electron microscopy revealed that the acrosome reaction started within 60 min at 37.degree.C and affected up to 80% of spermatozoa in 4 h. These membrane particles differed from those reported previously in other species in enzyme composition, function and organ of origin.