Castration induced browning in subcutaneous white adipose tissue in male mice
Castration induced browning in subcutaneous white adipose tissue in male mice
复制标题
DOI：
10.1016/j.bbrc.2016.09.017
复制
发表时间：
2016-09-30
影响因子：
3.1
通讯作者：
Funaba, Masayuki
中科院分区：
文献类型：
--
作者：
Hashimoto, Osamu;Noda, Tatsuya;Funaba, Masayuki
我们证明，去势增强了雄性小鼠棕色脂肪组织 (BAT) 和皮下 (sc) 白色脂肪组织 (WAT) 中解偶联蛋白 1 (Ucp1)（一种生热蛋白）的表达。与假手术小鼠相比，阉割的雄性小鼠体温升高，体重增加减少。去势雄性小鼠中 BAT Ucp1 mRNA 表达量显着高于假手术小鼠。组织学上，在去势的腹股沟 scWAT 中观察到具有多房脂肪滴的细胞。免疫组织化学染色显示这些细胞与抗 Ucp1 抗体呈阳性反应。与假手术的 WAT 相比，去势 WAT 中腹股沟淋巴结附近的 Ucp1 阳性区域更广泛。高脂饮食喂养抑制了 scWAT 中去势诱导的 Ucp1 上调。这些发现表明，BAT 激活和 scWAT 褐变产生的产热有助于去势诱导的体重增加抑制。然而，考虑到去势的效果因高脂肪饮食消耗而减弱，对去势反应的产热刺激受到慢性营养过剩的抑制。 (C) 2016 Elsevier Inc. 保留所有权利。
We demonstrated that castration enhanced the expression of uncoupling protein 1 (Ucp1), a thermogenic protein, in brown adipose tissue (BAT) and subcutaneous (sc) white adipose tissue (WAT) in male mice. Castration of male mice increased body temperature and reduced body weight gain compared with those of sham-operated mice. BAT Ucp1 mRNA expression in castrated male mice was significantly higher than that in sham-operated mice. Histologically, cells with multilocular fat droplets were observed in the castrated inguinal scWAT. Immunohistochemical staining revealed that these cells positively reacted with the anti-Ucp1 antibody. The Ucp1-positive area near the inguinal lymph node in the castrated WAT was extensive compared with that of the sham-operated WAT. Castration-induced Ucp1 up-regulation in scWAT was suppressed by high-fat diet feeding. These findings suggest that thermogenesis by BAT activation and scWAT browning contribute to castration-induced inhibition of body weight gain. However, considering that the effect of castration was blunted by high-fat diet consumption, thermogenesis stimulation in response to castration is inhibited by chronic over-nutrition. (C) 2016 Elsevier Inc. All rights reserved.