Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration.
Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration.
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DOI：
10.1002/0471142301.ns0417s57
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发表时间：
2011-10
影响因子：
--
通讯作者：
Samulski, Richard Jude
中科院分区：
文献类型：
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作者：
Gray, Steven J;Choi, Vivian W;Asokan, Aravind;Haberman, Rebecca A;McCown, Thomas J;Samulski, Richard Jude
腺相关病毒是一种非致病性的人类病毒，由于其对许多不同细胞类型的高感染效率及其持续存在并导致长期基因表达的能力，已被开发成基因递送载体。本单元描述了产生高滴度，研究级，无腺病毒的重组单链和自身互补的腺相关病毒在各种血清型的有效方法，沿着的方法来量化的病毒载体。提供了两种用于病毒载体递送到啮齿动物脑和脊髓中以及用于GFP的转基因表达的组织学检测的详细方法。
Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors. Two detailed methods are provided for viral vector delivery into the rodent brain and spinal cord, and for histological detection of transgene expression of GFP.