Porous silicon-graphene oxide core-shell nanoparticles for targeted delivery of siRNA to the injured brain.
Porous silicon-graphene oxide core-shell nanoparticles for targeted delivery of siRNA to the injured brain.
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DOI：
10.1039/c6nh00082g
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发表时间：
2016-09-01
影响因子：
9.7
通讯作者：
Sailor MJ
中科院分区：
文献类型：
--
作者：
Joo J;Kwon EJ;Kang J;Skalak M;Anglin EJ;Mann AP;Ruoslahti E;Bhatia SN;Sailor MJ
我们报道了一种基于纳米颗粒的RNAi递送平台的合成、表征和评估，该平台可以保护siRNA有效载荷免受核酸酶诱导的降解，并有效地将它们递送到靶细胞。该纳米载体是基于可生物降解的介孔硅纳米颗粒(PSiNPs)，其中纳米颗粒的空隙中负载有siRNA，纳米颗粒被包裹在氧化石墨烯纳米片(GO-pSiNPs)中。氧化石墨烯胶囊剂将寡核苷酸有效载荷的体外释放延迟了3倍。当与狂犬病病毒糖蛋白(RVG)衍生的靶向多肽结合时，纳米粒显示出2倍的细胞摄取和基因沉默。将纳米颗粒静脉注射到脑损伤小鼠体内，会导致大量聚集，特别是在损伤部位。
We report the synthesis, characterization, and assessment of a nanoparticle-based RNAi delivery platform that protects siRNA payloads against nuclease-induced degradation and efficiently delivers them to target cells. The nanocarrier is based on biodegradable mesoporous silicon nanoparticles (pSiNPs), where the voids of the nanoparticles are loaded with siRNA and the nanoparticles are encapsulated with graphene oxide nanosheets (GO-pSiNPs). The graphene oxide encapsulant delays release of the oligonucleotide payloads in vitro by a factor of 3. When conjugated to a targeting peptide derived from the rabies virus glycoprotein (RVG), the nanoparticles show 2-fold greater cellular uptake and gene silencing. Intravenous administration of the nanoparticles into brain-injured mice results in substantial accumulation specifically at the site of injury.