Time lapse analysis of root elongation rates of rice and sorghum during the day and night
Time lapse analysis of root elongation rates of rice and sorghum during the day and night
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DOI：
10.1006/anbo.1998.0611
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发表时间：
1998-05-01
期刊：
影响因子：
4.2
通讯作者：
Kono, Y
中科院分区：
文献类型：
--
作者：
Iijima, M;Oribe, Y;Kono, Y
本文介绍了一种技术，以监测根伸长速率（RER）每小时数天，并在RER在白天和夜间的变化。水稻（Oryza sativa L.）和高粱（Sorghum bicolor Moench）在置于生长室内的根盒中生长，生长室内设定为25 ℃，光周期为12小时。种子根轴夹在透明的丙烯酸板和滤纸之间，放置在壤质砂土上。根在昏暗的绿色光下使用连接到时间推移录像机的CCD照相机拍照。根的环境，包括温度，光，营养，水和空气供应，精确控制和保持恒定。粗面内质网在高粱中每小时波动，在水稻中波动更大。最大RERs比最小速率快1.4至4.4个时间。当温度相同时，白天和晚上的RERs没有统计学差异。(C)1998年《植物学年鉴》（Annals of Botany Company）
This paper describes a technique to monitor the root elongation rate (RER) per hour for several days, and variation in RER during the day and night. Rice (Oryza sativa L.) and sorghum (Sorghum bicolor Moench) were grown in root boxes placed inside a growth chamber set at 25 degrees C with a 12 h photoperiod. Seminal root axes were sandwiched between a transparent acrylic board and filter paper placed on a loamy sand soil. The roots were photographed under dim green light using a CCD camera connected to a time lapse video recorder. The environment of the root, including temperature, light, nutrient, water and air supply, was controlled precisely and maintained constant. RER fluctuated hourly in sorghum and to a greater extent in rice. Maximum RERs were 1.4 to 4.4 limes faster than minimum rates. RERs during the day and night did not differ statistically when temperatures were the same. (C) 1998 Annals of Botany Company.