Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts.
Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts.
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光致发光阳离子碳点作为质粒 SOX9 的有效非病毒传递和成纤维细胞的软骨形成
DOI：
10.1038/s41598-018-25330-x
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发表时间：
2018-05-04
影响因子：
4.6
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Cao X;Wang J;Deng W;Chen J;Wang Y;Zhou J;Du P;Xu W;Wang Q;Wang Q;Yu Q;Spector M;Yu J;Xu X
随着对更高基因载体性能的需求不断增加，多功能载体可以极大地简化用于疾病治疗的基因递送;然而，目前的非病毒载体缺乏自跟踪能力。在这里，一种新型的，双功能的阳离子碳点（CD），通过一步，微波辅助热解的精氨酸和葡萄糖，已被用作自成像剂和非病毒基因载体的成纤维细胞的软骨形成。该阳离子CD可与模型基因质粒SOX 9（pSOX 9）缩合形成超小（10-30 nm）纳米颗粒，具有高溶解度、荧光可调、产率高、细胞毒性低和生物相容性好等优点。MTT试验表明，与Lipofectamine 2000和PEI（25 kDa）相比，CD/pSOX 9纳米颗粒对小鼠胚胎成纤维细胞（MEF）的细胞毒性很小。重要的是，具有可调荧光的CD/pSOX 9纳米颗粒不仅能够实现纳米颗粒的细胞内跟踪，而且还可以以显著高的效率成功地将pSOX 9递送到MEFs中。此外，CD/pSOX 9纳米颗粒介导的MEFs转染显示出明显的软骨分化。总而言之，这些发现表明，本研究中制备的CD可以作为自成像和有效非病毒基因递送的双功能试剂的典范。
With the increasing demand for higher gene carrier performance, a multifunctional vector could immensely simplify gene delivery for disease treatment; nevertheless, the current non- viral vectors lack self-tracking ability. Here, a type of novel, dual-functional cationic carbon dots (CDs), produced through one-step, microwave-assisted pyrolysis of arginine and glucose, have been utilized as both a self-imaging agent and a non-viral gene vector for chondrogenesis from fibroblasts. The cationic CDs could condense the model gene plasmid SOX9 (pSOX9) to form ultra-small (10–30 nm) nanoparticles which possessed several favorable properties, including high solubility, tunable fluorescence, high yield, low cytotoxicity and outstanding biocompatibility. The MTT assay indicated that CDs/pSOX9 nanoparticles had little cytotoxicity against mouse embryonic fibroblasts (MEFs) compared to Lipofectamine2000 and PEI (25 kDa). Importantly, the CDs/pSOX9 nanoparticles with tunable fluorescence not only enabled the intracellular tracking of the nanoparticles, but also could successfully deliver the pSOX9 into MEFs with significantly high efficiency. Furthermore, the CDs/pSOX9 nanoparticles-mediated transfection of MEFs showed obvious chondrogenic differentiation. Altogether, these findings demonstrated that the CDs prepared in this study could serve as a paradigmatic example of the dual-functional reagent for both self-imaging and effective non-viral gene delivery.
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影响因子：
3.1
作者：
Cao, Xia;Deng, Wenwen;Yu, Jiangnan
通讯作者：
Yu, Jiangnan
影响因子：
9.7
作者：
Segovia, Nathaly;Dosta, Pere;Borros, Salvador
通讯作者：
Borros, Salvador
影响因子：
--
作者：
Ikeda, T;Kamekura, S;Chung, UI
通讯作者：
Chung, UI
影响因子：
8
作者：
Deng WW;Cao X;Wang M;Qu R;Su WY;Yang Y;Wei YW;Xu XM;Yu JN
通讯作者：
Yu JN
影响因子：
10.5
作者：
Kadaja M;Keyes BE;Lin M;Pasolli HA;Genander M;Polak L;Stokes N;Zheng D;Fuchs E
通讯作者：
Fuchs E