A Mesoscale 3D Culture System for Native and Engineered Biphasic Tissues: Application to the Osteochondral Unit.
A Mesoscale 3D Culture System for Native and Engineered Biphasic Tissues: Application to the Osteochondral Unit.
复制标题
DOI：
10.1007/978-1-0716-1693-2_16
复制
发表时间：
2021-09
影响因子：
--
通讯作者：
Irene Chiesa;R. Di Gesù;Kalon J. Overholt;R. Gottardi
中科院分区：
文献类型：
--
作者：
Irene Chiesa;R. Di Gesù;Kalon J. Overholt;R. Gottardi
界面组织是一种具有复杂层状结构的功能梯度组织，其体外培养和复制面临着巨大的挑战。在这里，我们描述了3D打印双室生物反应器的设计和操作，作为双相天然或工程化骨软骨组织的培养系统。生物反应器被设计成潜在地容纳各种界面组织，并且通过在保持组织隔室直接接触的同时创建两个单独的微环境来实现组织串扰的精确研究。
Interface tissues are functionally graded tissues characterized by a complex layered structure, which therefore present a great challenge to be reproduced and cultured in vitro. Here, we describe the design and operation of a 3D printed dual-chamber bioreactor as a culturing system for biphasic native or engineered osteochondral tissues. The bioreactor is designed to potentially accommodate a variety of interface tissues and enables the precise study of tissue crosstalk by creating two separate microenvironments while maintaining the tissue compartments in direct contact.