APPLICATION OF FAST ATOM BOMBARDMENT WITH TANDEM MASS-SPECTROMETRY AND LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY TO THE ANALYSIS OF ACYLCARNITINES IN HUMAN-URINE, BLOOD, AND TISSUE
APPLICATION OF FAST ATOM BOMBARDMENT WITH TANDEM MASS-SPECTROMETRY AND LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY TO THE ANALYSIS OF ACYLCARNITINES IN HUMAN-URINE, BLOOD, AND TISSUE
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DOI：
10.1016/0003-2697(89)90441-7
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发表时间：
1989-08-01
影响因子：
2.9
通讯作者：
INOUE, F
中科院分区：
文献类型：
--
作者：
MILLINGTON, DS;NORWOOD, DL;INOUE, F
使用三重四极杆质谱仪上的示踪离子扫描功能，通过快原子轰击质谱法在目标基质中检测到浓度为1纳摩尔/克或以下的酰基肉毒碱。已知代谢紊乱患者的酰基卡尼汀谱与先前获得的数据一致。假定酰基肉毒碱信号证实在一种情况下，通过管理稳定的同位素标记的肉毒碱，迅速与内源性池平衡。连续流动系统的增加使得无需操作员干预的快速连续分析成为可能，这表明分析程序自动化的潜力。微型LC柱的结合使得能够对选定的患者样品进行在线液相色谱/质谱分析。大规模筛查和定量分析尿液或血液中的诊断酰基肉毒碱是不可行的。
Using a precursor-ion scan function on a triple quadrupole mass spectrometer, acylcarnitines were detected in the target matrices at or below concentrations of 1 nmol per gram by fast atom bombardment mass spectrometry. Acylcarnitine profiles from patients with known metabolic disorders were consistent with previously acquired data. Putative acylcarnitine signals were confirmed in one case by administration of stable isotope-labeled carnitine, which equilbrated rapidly with the endogenous pool. The addition of a continuous flow system enabled rapid sequential analysis without operator intervention, indicating the potential for automation of the analytical procedure. Incorporation of a micro-LC column enabled on-line liquid chromatographic/mass spectrometric analysis of selected patient samples. Large-scale screening and quantitative analysis of urine or blood for diagnostic acylcarnitines are not practicable.