Chronic restraint stress decreases the expression of glutathione S-transferase pi2 in the mouse hippocampus
Chronic restraint stress decreases the expression of glutathione S-transferase pi2 in the mouse hippocampus
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DOI：
10.1016/j.brainres.2006.03.062
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发表时间：
2006-05-23
期刊：
影响因子：
2.9
通讯作者：
Young, L. Trevor
中科院分区：
文献类型：
--
作者：
Ejchel-Cohen, Tatiana F.;Wood, Gwendolyn E.;Young, L. Trevor
慢性束缚应激对小鼠海马结构和功能的影响。小鼠每天进行约束3周，海马中的基因表达进行了比较，控制使用大规模的cDNA微阵列。我们发现444个基因的差异表达，并进一步分析6个基因的实时逆转录PCR证实，其中3个下调胁迫。这3个基因，生长因子受体结合蛋白2（Grb 2），磷脂酰肌醇-4-磷酸5-激酶，1型β（PipSk 1b），谷胱甘肽S-转移酶，π 2（Gstp 2），也进行了原位杂交分析。Gstp 2表达下调可导致海马CA 1、CA 3区锥体细胞和齿状回颗粒层氧化损伤增加，导致结构和功能损伤。这些区域受到压力的影响，我们的研究结果可能有助于进一步了解与压力相关的疾病发生的机制。(c)2006 Elsevier B. V.保留所有权利。
Chronic restraint stress in mice affects hippocampal structure and function. Mice were subjected to daily restraint for 3 weeks, and gene expression in hippocampus was compared to controls using large-scale cDNA microarrays. We found that 444 genes were differentially expressed, and further analysis of 6 genes by real-time reverse transcription PCR confirmed that 3 of them were downregulated by stress. These 3 genes, growth factor receptor-bound protein 2 (Grb2), phosphatidylinositol-4-phosphate 5-kinase, type 1 beta (PipSk1b), and glutathione S-transferase, pi2 (Gstp2), were also analyzed by in situ hybridization. The downregulation of Gstp2 may induce an increase of oxidative damage in the pyramidal cells of the CA1 and CA3 regions and granular layer of the dentate gyrus, leading to structural and functional damage. Those regions are affected by stress, and our results could help understand further the mechanisms involved in the occurrence of stress-related disorders. (c) 2006 Elsevier B.V. All rights reserved.