PulseNet and the Changing Paradigm of Laboratory-Based Surveillance for Foodborne Diseases
PulseNet and the Changing Paradigm of Laboratory-Based Surveillance for Foodborne Diseases
复制标题
DOI：
10.1177/0033354919881650
复制
发表时间：
2019-11-01
影响因子：
3.3
通讯作者：
Gerner-Smidt, Peter
中科院分区：
文献类型：
--
作者：
Kubota, Kristy A.;Wolfgang, William J.;Gerner-Smidt, Peter
PulseNet是国家食源性疾病监测分子亚型网络，成立于1996年，由美国疾病控制和预防中心、美国农业部、食品安全和检验局、美国食品和药物管理局、4个州公共卫生实验室和公共卫生实验室协会合作建立。该网络已扩大到包括83个州，地方和食品监管公共卫生实验室。2016年，据估计PulseNet每年帮助预防约270，000起食源性疾病。PulseNet正在向全基因组测序（WGS）转型，它提供了比脉冲场凝胶电泳（PFGE）更好的区分能力和精度。WGS改进了爆发集群的检测，可以取代许多传统的参考识别和表征方法。本文强调了公共卫生实验室在改变PulseNet的监测方面所做的贡献，并介绍了这种转变如何改变地方和国家的监测实践。我们的数据表明，WGS是更好地识别集群比PFGE，特别是克隆生物，如沙门氏菌肠道。在美国，PulseNet实施WGS进行食源性疾病监测时，需要为集群后续行动制定优先计划，并为公共卫生实验室和流行病学部门提供额外资源。
PulseNet, the National Molecular Subtyping Network for Foodborne Disease Surveillance, was established in 1996 through a collaboration with the Centers for Disease Control and Prevention; the US Department of Agriculture, Food Safety and Inspection Service; the US Food and Drug Administration; 4 state public health laboratories; and the Association of Public Health Laboratories. The network has since expanded to include 83 state, local, and food regulatory public health laboratories. In 2016, PulseNet was estimated to be helping prevent an estimated 270 000 foodborne illnesses annually. PulseNet is undergoing a transformation toward whole-genome sequencing (WGS), which provides better discriminatory power and precision than pulsed-field gel electrophoresis (PFGE). WGS improves the detection of outbreak clusters and could replace many traditional reference identification and characterization methods. This article highlights the contributions made by public health laboratories in transforming PulseNet's surveillance and describes how the transformation is changing local and national surveillance practices. Our data show that WGS is better at identifying clusters than PFGE, especially for clonal organisms such as Salmonella Enteritidis. The need to develop prioritization schemes for cluster follow-up and additional resources for both public health laboratory and epidemiology departments will be critical as PulseNet implements WGS for foodborne disease surveillance in the United States.