Amino acid residues in the CDC25 guanine nucleotide exchange factor critical for interaction with Ras.
Amino acid residues in the CDC25 guanine nucleotide exchange factor critical for interaction with Ras.
复制标题
CDC25 鸟嘌呤核苷酸交换因子中的氨基酸残基对于与 Ras 的相互作用至关重要。
DOI：
10.1128/mcb.14.12.8117-8122.1994
复制
发表时间：
1994
影响因子：
5.3
通讯作者：
Broek,D
中科院分区：
文献类型：
--
作者：
Park,W;Mosteller,RD;Broek,D
Previously we found that negatively charged residues at positions 62, 63, and 69 of H-Ras are involved in binding to the CDC25 guanine nucleotide exchange factor (GEF). Using site-directed mutagenesis, we have changed conserved, positively charged residues of CDC25GEFto glutamic acid. We find the nonfunctional CDC25R1374Emutant and the nonfunctional H-RasE63Kmutant cooperate in suppression of the loss ofCDC25function inSaccharomyces cerevisiae.Also, peptides corresponding to residues 1364 to 1383 of CDC25GEFinhibit interaction between GEFs and H-Ras. We propose that residues 1374 of CDC25GEFand 63 of H-Ras form an ion pair and that when this ion pair is reversed, functional interaction can still occur.
