Mutation scanning-coupled analysis of haplotypic variability in mitochondrial DNA regions reveals low gene flow between human and porcine Ascaris in endemic regions of China
Mutation scanning-coupled analysis of haplotypic variability in mitochondrial DNA regions reveals low gene flow between human and porcine Ascaris in endemic regions of China
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DOI：
10.1002/elps.200500276
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发表时间：
2005-11-01
期刊：
影响因子：
2.9
通讯作者：
Gasser, RB
中科院分区：
文献类型：
--
作者：
Peng, WD;Yuan, K;Gasser, RB
对中国6个省蛔虫种群内及种群间的单倍型变异进行了调查。从人类和猪蛔虫个体的总基因组DNA样本（n bbb720）中，通过PCR扩增出细胞色素c氧化酶亚基1 （cox1）和NADH脱氢酶亚基1 （nad1）基因的线粒体DNA区域，并进行突变扫描和随后的选择性测序。对于cox1，在人蛔虫中记录了10个不同的电泳谱，在猪蛔虫中记录了相同的电泳谱，其中一个在两种宿主物种中都是常见的。nad1在人蛔虫和猪蛔虫中分别检测到11个和15个不同的基因图谱。通过测序确定了所有单倍型（pcox1为20个，pnad1为26个），估计了它们在两种宿主物种和6个省中的频率。对于每个线粒体区域，不同单倍型的频率差异很大，这取决于宿主物种和地理来源。序列数据（代表每个线粒体位点的所有单倍型）的f统计分析表明，人蛔虫和猪蛔虫之间的基因流动受限，并支持先前分子流行病学调查的结论，即猪不是流行地区人类蛔虫感染的重要来源。
Haplotypic variation within and among the Ascaris populations representing six provinces in China was investigated. Mitochondrial DNA regions in the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes were amplified by PCR from total genomic DNA samples (n > 720) from Ascaris individuals from humans and pigs, and subjected to mutation scanning and subsequent selective sequencing. For the cox1, ten different electrophoretic profiles were recorded for human Ascaris, and the same number for pig Ascaris, one of them being common to both host species. For the nad1, 11 different profiles were detected for human Ascaris, and 15 for pig Ascaris. Having defined all haplotypes (20 for pcox1 and 26 for pnad1) by sequencing, their frequencies were estimated in each of the two host species and each of the six provinces. For each mitochondrial region, the frequency of the different haplotypes varied considerably, depending on host species and geographical origin. Analysis of the sequence data (representing all haplotypes for each mitochondrial locus) by F-statistics indicated restricted gene flow between human Ascaris and pig Ascaris, and supported the conclusions from previous molecular epidemiological investigations that pigs are not a significant source of Ascaris infection in humans in endemic regions.