klavier (klv), a novel hypernodulation mutant of Lotus japonicus affected in vascular tissue organization and floral induction.
klavier (klv), a novel hypernodulation mutant of Lotus japonicus affected in vascular tissue organization and floral induction.
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DOI：
10.1111/j.1365-313x.2005.02543.x
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发表时间：
2005-10
期刊：
影响因子：
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通讯作者：
Erika Oka-Kira;Kumiko Tateno;K. Miura;T. Haga;M. Hayashi;K. Harada;Shusei Sato;S. Tabata;N. Sh
中科院分区：
文献类型：
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作者：
Erika Oka-Kira;Kumiko Tateno;K. Miura;T. Haga;M. Hayashi;K. Harada;Shusei Sato;S. Tabata;N. Sh
用氦离子束辐照百脉根Miyakojima MG-20，获得一个新的超增殖突变株系。这种突变体，命名为klavier（klv），其根上密布着小结节。klv的凝集带明显宽于野生型。嫁接实验表明klv在长距离茎-根自动调节机制中受损。因此，芽基因型被认为是负责负调控根瘤发育的KLV。KLV的结瘤表现出比野生型更高的耐氮性（KNO 3），这是一个共同的特点，hyperemulating突变体。klv突变体除了根瘤数增加外，近轴叶表面的叶脉凸起，显着延迟开花和矮化。显微镜检查发现，它们是不连续的。在klv突变体中还经常观察到其它表型，如扁茎、花数增加和雌蕊分叉。在这些表型中，过度生长、异常叶脉形成和显着延迟开花均以单基因和隐性方式连锁，表明这些表型由单一突变或紧密连锁突变引起。KLV定位在1号染色体长臂上0.29 cM以内。
A novel hypernodulation mutant line was isolated from Lotus japonicus Miyakojima MG-20 by irradiation with a helium ion beam. This mutant, named klavier (klv), had roots that were densely covered with small nodules. The nodulation zone of klv was significantly wider than that of the wild type. Grafting experiments showed that klv is impaired in the long-distance shoot-to-root autoregulatory mechanism. Thus the shoot genotype was found to be responsible for the negative regulation of nodule development by KLV. Nodulation of klv showed a higher tolerance to nitrogen (KNO3) than the wild type, which is a common feature of hypernodulating mutants. In addition to an increased number of nodules, the klv mutant showed convex leaf veins on the adaxial leaf surface, markedly delayed flowering and dwarf phenotypes. Microscopic examination of the leaf veins revealed that they were discontinuous. Other phenotypes such as fasciated stems, increased number of flowers and bifurcated pistils were also frequently observed in the klv mutant. Among these phenotypes, hypernodulation, aberrant leaf vein formation and significantly delayed flowering were all linked in a monogenic and recessive manner, indicating that these phenotypes are caused by either a single mutation, or tightly linked mutations. KLV was mapped within 0.29 cM on the long arm of chromosome 1.