Precise Targeting of Single Microelectrodes to Orientation Pinwheel Centers.
Precise Targeting of Single Microelectrodes to Orientation Pinwheel Centers.
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DOI：
10.21769/bioprotoc.3643
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发表时间：
2020-06
期刊：
影响因子：
0.8
通讯作者：
Xuemei Song;Ming Li;Tao Xu;D. Hu;A. Roe
中科院分区：
文献类型：
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作者：
Xuemei Song;Ming Li;Tao Xu;D. Hu;A. Roe
在哺乳动物视觉系统中，视觉形式感知的早期阶段开始于初级视皮层（V1）中的方向选择神经元。在许多物种（包括人类、猴子、树鼩、猫和雪貂）中，这些神经元都是以风车状的方向柱组织起来的。为了研究方向风车内部的功能组织，精确定位方向风车子域是非常重要的。因此，我们开发了一种技术，以提供一个定量的确定风车中心（PC）的位置。以前的研究仅仅依赖于皮质表面的血管图像来引导电极穿透到定向图中的PC。然而，使用这种方法仍然存在相当大的空间误差。在本研究中，我们提高了目标PC的准确性，通过确保电极的平行性，并通过利用局部场电位（LFP）记录在或附近的光学确定的位置的取向调整。
In the mammalian visual system, early stages of visual form perception begin with orientation selective neurons in primary visual cortex (V1). In many species (including humans, monkeys, tree shrews, cats, and ferrets), these neurons are organized in pinwheel-like orientation columns. To study the functional organization within orientation pinwheels, it is important to target pinwheel subdomains precisely. We therefore developed a technique to provide a quantitative determination of the location of pinwheel centers (PCs). Previous studies relied solely on blood vessel images of the cortical surface to guide electrode penetrations to PCs in orientation maps. However, considerable spatial error remained using this method. In the present study, we improved the accuracy of targeting PCs by ensuring perpendicularity of electrodes and by utilizing the orientation tuning of local field potentials (LFP) recorded at or near the optically determined positions.