Characterization of γδ regulatory T cells from peripheral blood in patients with multiple myeloma
Characterization of γδ regulatory T cells from peripheral blood in patients with multiple myeloma
复制标题
多发性骨髓瘤患者外周血中 γ δ 调节性 T 细胞的特征
DOI：
10.1016/j.bbrc.2016.10.098
复制
发表时间：
2016-11-25
影响因子：
3.1
通讯作者：
Liu, Qifa
中科院分区：
文献类型：
--
作者：
Ma, Yongyong;Lei, Huyi;Liu, Qifa
γδ调节性T细胞能够抑制参与抗原特异性免疫反应的T细胞的激活和功能。本研究旨在探讨 γ δ 调节性 T 细胞在抑制多发性骨髓瘤 (MM) 患者抗肿瘤免疫反应中的潜在作用。我们测量了健康捐献者、最近诊断为 MM 的患者以及缓解队列中的 MM 患者外周血中 γ δ T 细胞的水平、分布和克隆扩增的 TCR V γ 和 V δ T 细胞。此外，我们评估了γδ调节性T细胞抑制CD4-FCD25-T细胞增殖的能力，并检测了免疫调节相关分子的表达。我们发现多发性骨髓瘤患者外周血中的γδ调节性T细胞水平显着高于健康供者。 MM 和健康供体中 γ δ T 调节细胞功能的比较显示出对 T 细胞增殖的类似抑制作用。此外，与健康供者相比，MM患者中TLR8的表达水平显着升高，而MM患者与健康供者中Foxp3、CD25、CTLA4、GITR、GATA3和Tbet的表达水平无显着差异。综上所述，我们的研究揭示了 γδ 调节性 T 细胞在抑制 MM 患者抗肿瘤免疫反应中的潜在作用。 (C) 2016 Elsevier Inc. 保留所有权利。
gamma delta regulatory T cells are able to inhibit the activation and function of T cells involved in antigen-specific immune responses. This study aimed to investigate the potential role of gamma delta regulatory T cells in inhibiting anti-tumor immune responses in patients diagnosed as multiple myeloma (MM). We measured the levels of gamma delta T cells, the distribution and clonally amplified TCR V gamma and V delta T cells in peripheral blood of healthy donors, patients recently diagnosed with MM, and MM patients in remission cohorts. In addition, we evaluated the ability of gamma delta regulatory T cells to inhibit the proliferation of CD4-FCD25- T cells and detected the expression of immunoregulatory-associated molecules. We found that the levels of gamma delta regulatory T cells from the peripheral blood in patients of MM were significantly higher than those in healthy donors. Comparison of gamma delta T regulatory cells function in MM and healthy donors showed similarly inhibitory effects on the proliferation of T cells. Additionally, TLR8 expression level increased significantly in MM patients compared to healthy donors, while the expression levels of Foxp3, CD25, CTLA4, GITR, GATA3 and Tbet in MM patients and healthy donors showed no significant difference. Taken together, our study reveals the potential role of gamma delta regulatory T cells in inhibiting anti-tumor immune responses in MM patients. (C) 2016 Elsevier Inc. All rights reserved.