Time‐dose‐mortality modelling and virulence indices for six strains of Verticillium lecanii against sweetpotato whitefly, Bemisia tabaci (Gennadius)
Time‐dose‐mortality modelling and virulence indices for six strains of Verticillium lecanii against sweetpotato whitefly, Bemisia tabaci (Gennadius)
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DOI：
10.1111/j.1439-0418.2004.00879.x
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发表时间：
2004-08
影响因子：
1.9
通讯作者：
Liande Wang;Jian Huang;Minsheng You;B. Liu
中科院分区：
文献类型：
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作者：
Liande Wang;Jian Huang;Minsheng You;B. Liu
对6株蜡蚧轮枝菌Verticillium lecanii（Zimmermann）Viégas进行了生物活性测定。为了接种，将甘薯幼苗上的三龄粉虱批次浸入浓度为IO 3至IO 7分生孢子/ml的五种剂量的分生孢子悬浮液中。每次剂量为150-250 μ g。将果蝇保持在25°C、95%RH、16：8（L：D）的光相下，并每天观察死亡率。基于Hosmer-Lemeshow检验，通过互补log-log（CLL）时间-剂量-死亡率模型分析了8天内所得数据，同时分析了6种蜡蚧弧菌菌株的5种浓度的时间-剂量趋势。利用模型参数估算了6个菌株对B的毒力指数（LC 50值）。烟粉虱根据拟合的时间-剂量-死亡率关系和毒力指数，对6株蜡蚧轮枝菌对B.塔巴伊进行了比较。结果表明，加拿大进口菌株V16063和国内菌株V3450、Vp 28均来源于B.烟粉虱和蚧虫的毒力最强，LC_（50）分别为2.57 × 105、6.03 × 105和6.03 × 105。
Bioassays of six strains of Verticillium lecanii (Zimmermann) Viégas were conducted with the sweet potato whitefly, Bemisia tabaci. For inoculation, batches of third‐instar whitefly nymphs on sweetpotato seedlings were immersed in conidial suspensions of five dosages at concentrations from 103 to 107 conidia/ml. Each dosage was used to inoculate 150–250 nymphs. The nymphs were maintained at 25°C, under 95% RH, photo phase of 16 : 8 (L : D) and observed daily for mortality. The resulting data were analysed over 8 days by a complementary log‐log (CLL) time‐dose‐mortality model, based on the Hosmer–Lemeshow test, analysing the time‐dose trends for five concentrations of six strains of V. lecanii simultaneously. The parameters from the model were used to estimate the virulence indices (the values of LC50) of six strains against B. tabaci. Based on the time‐dose‐mortality relationships fitted and the virulence indices, the virulence of the six strains of V. lecanii for B. tabai was compared. Results indicated that the strain Vl6063 imported from Canada and the domestic strains V3450 and Vp28 derived from B. tabaci and a scale insect, respectively, were more virulent than the others with LC50 values of 2.57 × 105, 6.03 × 105 and 6.03 × 105, respectively.