Sulphated polysaccharides from Indian samples of Enteromorpha compressa (Ulvales, Chlorophyta):: Isolation and structural features
Sulphated polysaccharides from Indian samples of Enteromorpha compressa (Ulvales, Chlorophyta):: Isolation and structural features
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DOI：
10.1016/j.foodchem.2006.12.048
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Ray, Bimalendu
中科院分区：
文献类型：
--
作者：
Chattopadhyay, Kausik;Mandal, Pinaki;Ray, Bimalendu
通过依次用水和碱提取，从印度产的浒苔样本中提取出富含杂聚糖和木葡聚糖的组分，产率为25%。这种杂聚糖是硫酸化的，表观分子量为55 kDa。对这种多糖的化学结构分析显示其为分支结构，含有1,4 - 和1,2,4 - 连接的鼠李糖 - 3 - 硫酸酯、1,4 - 连接的葡萄糖、1,3 - 和1,6 - 连接的半乳糖、1,4 - 和末端连接的葡萄糖醛酸以及1,4 - 连接的木糖（其O - 2位部分硫酸化）。化学和光谱分析表明，用4M氢氧化钾提取的半纤维素组分含有一种不寻常的β - (1,4) - 连接的线性木葡聚糖。通过基质辅助激光解吸电离 - 飞行时间质谱（MALDI - TOF - MS）对酶水解产物及所得片段进行分析，结果表明这种线性聚合物含有部分硫酸化的Glc(3)Xyl(2)或Glc(4)Xyl(2)作为寡聚构建亚基。（c）2007爱思唯尔有限公司。保留所有权利。
Heteroglycan and xyloglucan rich fractions were extracted from Indian samples of Enteromorpha compressa in 25% yield by sequential extractions with water and alkali. This heteroglycan is sulfated and has an apparent molecular mass of 55 kDa. Chemical structural analysis of this polysaccharide revealed a branched structure having 1,4- and 1,2,4-linked rhamnose 3-sulphate, 1,4-linked glucose, 1,3- and 1,6- linked galactose, 1,4- and terminally linked glucuronic acid and 1,4-linked xylose partially sulfated on 0-2. Chemical and spectroscopic analysis showed that the 4-M KOH extracted hernicellulosic fraction contained an unusual beta-(1,4)-linked linear xyloglucan. Enzyme hydrolysis and analysis of the resulting fragments by matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) showed that this linear polymer contained partially sulfated Glc(3)Xyl(2) or Glc(4)Xyl(2) as oligomeric building subunits. (c) 2007 Elsevier Ltd. All rights reserved.