CHROMATOGRAPHIC PURIFICATION OF NATURAL LYCOPENE
CHROMATOGRAPHIC PURIFICATION OF NATURAL LYCOPENE
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DOI：
10.1021/jf00042a012
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发表时间：
1994-06-01
影响因子：
6.1
通讯作者：
HEINONEN, IM
中科院分区：
文献类型：
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作者：
HAKALA, SH;HEINONEN, IM
天然番茄红素的分离方法分三步建立。首先，用石油醚从番茄泥中提取类胡萝卜素；然后，用硅胶小柱从除番茄红素以外的所有类胡萝卜素中预纯化类胡萝卜素。采用半制备型高效液相色谱柱(Mu Bondapak C-18)对番茄红素进行了三次纯化。用Zorbax ODS色谱柱进行分析，每步检测番茄红素的纯度和回收率。采用乙腈-二氯甲烷-甲醇(45：10：45)的混合溶剂。用二极管阵列检测确定了番茄红素峰的肩峰。此外，还进行了峰纯度的光谱扫描。提取后，从10g番茄泥中回收番茄红素30 mg，番茄红素含量占类胡萝卜素总量的87%。固相萃取后，番茄红素残留量为21 mg，占总类胡萝卜素的93%，占全反式番茄红素的80%。在第一次和第三次高效液相分离后，番茄红素的含量分别从9 mg下降到6 mg。用该方法从10g番茄泥中分离出6 mg番茄红素。分离得到的天然番茄红素含有约20%的顺式番茄红素和3%的叶黄素。
The natural lycopene isolation method was developed in three steps. First, carotenoids were extracted from tomato puree with petroleum ether; next, the carotenoid extract was prepurified from all carotenoids except lycopene with solid-phase extraction (SPE) using silica cartridges. Lycopene was then further purified three times with semipreparative high-performance liquid chromatography (HPLC) using a mu Bondapak C-18 column. The purity and recovery of the lycopene were checked after each step with analytical HPLC using a Zorbax ODS column. A solvent mixture of acetonitrile-dichloromethane-methanol (45:10:45) was used in all HPLC evaluations. The shoulder of the lycopene peak was identified with diode array detection. Also, spectral scans for peak purity were made. After extraction, 30 mg of lycopene was recovered from 10 g of tomato puree and the lycopene content amounted to 87% of the total carotenoids. After the SPE, 21 mg of the extracted lycopene remained, the lycopene content being 93% of total carotenoids and that of all-trans-lycopene 80%. The amount of lycopene after the first and third HPLC fractionations declined from 9 to 6 mg, respectively. With the method developed, 6 mg of lycopene was isolated from 10 g of tomato puree. However, the natural lycopene isolated contained about 20% lycopene cis isomers and 3% xanthophylls.