Enhancing cell seeding of scaffolds in tissue engineering through manipulation of hydrodynamic parameters
Enhancing cell seeding of scaffolds in tissue engineering through manipulation of hydrodynamic parameters
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DOI：
10.1016/j.jbiotec.2007.01.005
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发表时间：
2007-05-01
影响因子：
4.1
通讯作者：
Barabino, Gilda A.
中科院分区：
文献类型：
--
作者：
Bueno, Ericka M.;Laevsky, Gary;Barabino, Gilda A.
将细胞接种到生物相容性支架上是获得工程组织功能特性的决定性步骤。高效、快速且空间均匀的细胞接种可以提高工程组织模板的临床潜力。达到这些细胞接种要求的一种方法是通过生物反应器设计。在本研究中，牛软骨细胞接种（2.5，5.0或10.0万个细胞/支架）到聚乙醇酸支架内的波壁和旋转瓶生物反应器的流体动力学环境。以前的表征在这些生物反应器中培养的构建体附近的流体动力学环境表明，降低流动诱导的剪切应力以及增加的再循环和波壁生物反应器中的轴向流体速度的大小。在这里，我们报告了更有效和空间均匀的细胞接种在波壁生物反应器中，并在中间初始细胞密度（500万细胞每个支架）。这项研究构成了一个重要的一步，实现功能性组织工程植入物（i）增加我们的理解的影响，流体动力学参数的效率和空间分布的细胞附着到支架和生产的细胞外基质和（ii）引入一个全面的方法来调查的生物加工条件对组织形态和组成的影响。(c)2007 Elsevier B. V.保留所有权利。
The seeding of cells onto biocompatible scaffolds is a determinant step in the attainment of functional properties of engineered tissues. Efficient, fast and spatially uniform cell seeding can improve the clinical potential of engineered tissue templates. One way to approach these cell seeding requirements is through bioreactor design. In the present study, bovine chondrocytes were seeded (2.5, 5.0 or 10.0 million cells per scaffold) onto polyglycolic acid scaffolds within the hydrodynamic environments of wavy-walled and spinner flask bioreactors. Previous characterizations of the hydrodynamic environment in the vicinity of constructs cultivated in these bioreactors suggested decreased flow-induced shear stress as well as increased recirculation and magnitude of the axial fluid velocities in the wavy-walled bioreactor. Here we report more efficient and spatially uniform cell seeding in the wavy-walled bioreactor, and at intermediate initial cell densities (5 million cells per scaffold). This study constitutes an important step towards the achievement of functional tissue-engineered implants by (i) increasing our understanding of the influence of hydrodynamic parameters on the efficiency and spatial distribution of cell attachment to scaffolds and the production of extracellular matrix and (ii) introducing a comprehensive approach to the investigation of the effects of bioprocessing conditions on tissue morphology and composition. (c) 2007 Elsevier B.V. All rights reserved.