The G-protein γ subunit gpc-1 of the nematode C-elegans is involved in taste adaptation
The G-protein γ subunit gpc-1 of the nematode C-elegans is involved in taste adaptation
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DOI：
10.1093/emboj/21.5.986
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发表时间：
2002-03-01
期刊：
影响因子：
11.4
通讯作者：
Plasterk, RHA
中科院分区：
文献类型：
--
作者：
Jansen, G;Weinkove, D;Plasterk, RHA
秀丽线虫有两个异源三聚体G蛋白伽马亚基，GPC-1和GPC-2。虽然GPC-1在感觉神经元中特异表达，但它对气味或盐类的检测并不是必需的。为了测试GPC-1是否参与感觉可塑性，我们开发了一种水溶性化合物适应试验。野生动物在本试验中的行为证实，长时间暴露在盐中可以消除对这些化合物的化学吸引力。这个过程依赖于时间和浓度，部分是盐特定的和可逆的。相比之下，GPC-1突变动物在适应NaAc、NaCl和NH4Cl方面表现出明显的缺陷，但正常的野生型适应气味。另外两个与气味适应有关的基因，ADP-1和OSM-9，也参与了对盐的适应。我们的发现G蛋白、OSM-9和ADP-1参与了味觉适应，这为我们对这一过程提供了第一个分子洞察力。
Caenorhabditis elegans has two heterotrimeric G-protein gamma subunits, gpc-1 and gpc-2. Although GPC-1 is specifically expressed in sensory neurons, it is not essential for the detection of odorants or salts. To test whether GPC-1 is involved in sensory plasticity, we developed a water soluble compound adaptation assay. The behaviour of wild-type animals in this assay confirms that prolonged exposure to salts can abolish chemo-attraction to these compounds. This process is time and concentration dependent, partly salt specific and reversible. In contrast, gpc-1 mutant animals show clear deficits in their ability to adapt to NaAc, NaCl and NH4Cl, but normal wild-type adaptation to odorants. Two other loci previously implicated in odorant adaptation, adp-1 and osm-9, are also involved in adaptation to salts. Our finding that G proteins, OSM-9 and ADP-1 are involved in taste adaptation offer the first molecular insight into this process.