Hydrogen sulphide, a novel gasotransmitter involved in guard cell signalling
Hydrogen sulphide, a novel gasotransmitter involved in guard cell signalling
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DOI：
10.1111/j.1469-8137.2010.03465.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Lamattina, Lorenzo
中科院分区：
文献类型：
--
作者：
Garcia-Mata, Carlos;Lamattina, Lorenzo
硫化氢（H_2S）已被提出作为第三类气体变送器。在动物细胞中，H2S与几种生理过程有关。H_2S是动物和植物内源性合成H_2S的酶，具有L-Cys脱氢酶活性，可将L-Cys转化为H_2S、丙酮酸和氨，本文以蚕豆（Vicia faba）、拟南芥（Arabidopsis thaliana）和华勒凤仙（Impatiens walleriana）为材料，研究了H_2S对气孔运动的调节和脱落酸（阿坝）诱导气孔关闭的作用。本文报道了外源H_2S诱导气孔关闭，ATP结合盒（ABC）转运蛋白抑制剂格列本脲（glibenclamide）可减弱H_2S对气孔运动的影响，清除H_2S或抑制内源H_2S合成酶可部分阻断ABA依赖的气孔关闭; H_2S处理提高了RWC，保护植物免受干旱胁迫的伤害，这表明H_2S诱导气孔关闭并参与ABA依赖的信号转导，可能是通过调节保卫细胞中的ABC转运蛋白来实现的。
P>Hydrogen sulphide (H2S) has been proposed as the third gasotransmitter. In animal cells, H2S has been implicated in several physiological processes. H2S is endogenously synthesized in both animals and plants by enzymes with l-Cys desulphydrase activity in the conversion of l-Cys to H2S, pyruvate and ammonia.The participation of H2S in both stomatal movement regulation and abscisic acid (ABA)-dependent induction of stomatal closure was studied in epidermal strips of three plant species (Vicia faba, Arabidopsis thaliana and Impatiens walleriana). The effect of H2S on stomatal movement was contrasted with leaf relative water content (RWC) measurements of whole plants subjected to water stress.In this work we report that exogenous H2S induces stomatal closure and this effect is impaired by the ATP-binding cassette (ABC) transporter inhibitor glibenclamide; scavenging H2S or inhibition of the enzyme responsible for endogenous H2S synthesis partially blocks ABA-dependent stomatal closure; and H2S treatment increases RWC and protects plants against drought stress.Our results indicate that H2S induces stomatal closure and participates in ABA-dependent signalling, possibly through the regulation of ABC transporters in guard cells.