Differential subcellular localization and activity of kelch repeat proteins KLHDC1 and KLHDC2
Differential subcellular localization and activity of kelch repeat proteins KLHDC1 and KLHDC2
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DOI：
10.1007/s11010-006-9304-6
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发表时间：
2007-02-01
影响因子：
4.3
通讯作者：
Jin, Dong-Yan
中科院分区：
文献类型：
--
作者：
Chin, King-Tung;Xu, Hai-Tao;Jin, Dong-Yan
我们以前已经确定和表征人类KLHDC 2/HCLP-1，kelch重复蛋白，相互作用，并抑制转录因子LZIP。在这项研究中，我们确定并表征了一个名为KLHDC 1的KLHDC 2蛋白。KLHDC 1和KLHDC 2在氨基酸水平上有约50%的同源性，两个基因位点均定位于人类染色体14q21.3。KLHDC 1和KLHDC 2基因簇在从河豚到人类的脊椎动物中高度保守。这两种基因在骨骼肌中高度表达，但在各种其他组织中表达较弱。KLHDC 2主要存在于细胞核中，而KLHDC 1是一种细胞质蛋白。KLHDC 1和KLHDC 2都不与肌动蛋白结合。此外，KLHDC 1不能抑制LZIP/CREB 3介导的转录激活。因此，KLHDC 1和KLHDC 2在培养的哺乳动物细胞中具有不同的定位和活性。
We have previously identified and characterized human KLHDC2/HCLP-1, a kelch repeat protein that interacts with and inhibits transcription factor LZIP. In this study, we identified and characterized a paralog of KLHDC2 called KLHDC1. KLHDC1 and KLHDC2 share about 50% identity at the level of amino acid sequence and both gene loci localize to human chromosome 14q21.3. This cluster of KLHDC1 and KLHDC2 genes is highly conserved in vertebrates ranging from pufferfish to human. Both genes are expressed highly in skeletal muscle, but weakly in various other tissues. While KLHDC2 was predominantly found in the nucleus, KLHDC1 is a cytoplasmic protein. Neither KLHDC1 nor KLHDC2 binds to actin. In addition, KLHDC1 was unable to inhibit LZIP/CREB3-mediated transcriptional activation. Thus, KLHDC1 and KLHDC2 have differential localization and activity in cultured mammalian cells.