Antifungal resistance of candidal biofilms formed on denture acrylic in vitro
Antifungal resistance of candidal biofilms formed on denture acrylic in vitro
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DOI：
10.1177/00220345010800031101
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发表时间：
2001-03-01
影响因子：
7.6
通讯作者：
Ghannoum, MA
中科院分区：
文献类型：
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作者：
Chandra, J;Mukherjee, PK;Ghannoum, MA
义齿生物膜代表口服微生物的保护库。这些生物膜中念珠菌生物学的研究需要可靠的模型。白色念珠菌义齿生物膜的可再现模型。开发并用于确定两种临床上相关的白色念珠菌分离株针对4种抗真菌物的敏感性。白色念珠菌作为生物膜生长，表现出对两性霉素B，Nystatin，洗涤酰胺和氟康唑的耐药性，其代谢活性（50％RMA）的浓度降低了50％，其浓度为8、16、128，> 64 mug，> 64 mug，mug，> 64毫克，分别。相比之下，木板培养的白色木出具有易感性（在0.25、1.0、4.0和0.5毫克/mL的情况下，同一抗真菌剂的RMA为50％RMA）。总之，通过我们的生物膜模型获得的结果表明，与生物膜相关的白色念珠菌细胞与以浮游症形式生长的细胞相比具有对治疗牙齿牙齿性气孔炎的抗真菌性抗真菌性的抗性。
Denture biofilms represent a protective reservoir for oral microbes. The study of the biology of Candida in these biofilms requires a reliable model. A reproducible model of C. albicans denture biofilm. was developed and used to determine the susceptibility of two clinically relevant C. albicans isolates against 4 antifungals. C. albicans, growing as a biofilm, exhibited resistance to amphotericin B, nystatin, chlorhexidine, and fluconazole, with 50% reduction in metabolic activity (50% RMA) at concentrations of 8, 16, 128, and > 64 mug/mL, respectively. In contrast, planktonically cultured C albicans were susceptible (50% RMA for the same antifungals was obtained at 0.25, 1.0, 4.0, and 0.5 mug/mL, respectively). In conclusion, results obtained by means of our biofilm model show that biofilm-associated C. albicans cells, compared with cells grown in planktonic form, are resistant to antifungals used to treat denture stomatitis.